Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Epidemiology》 1999-02
Add to Favorite Get Latest Update

Study on double antigens sandwich Enzyme Immunoassay for detection of brucella specific antibodies in human and animals

LU Qifa *, ZHANG Wei, HAO Zongyu. * Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206  
Objective In order to develop practicable methods for serodiagnosis,surveillance and epidemiological surveys in human and animals Brucellosis. Methods A double antigens sandwich Enzyme Immunoassay (DAgS-EIA include DAgS-ELISA and DAgS-DIEA) have been developed under the basis of first production conjugate of Brucella antigen with horseradish peroxidase. Serum samples from diagnosed patients, suspected patients of Brucellosis and sheep infected with Brucella in Henan and Hebei provinces as well as from experimental animals of infected with Brucella in laboratory detected through DAgS-EIA,I-ELISA,RBPT and SAT tests. Results Of the diagnosed and suspected Brucellosis patients, positive rates of DAgS-ELISA,RBPT,I-ELISA,DAgS-DIEA and SAT were 60.0 %- 62.9 %, 48.6 %- 58.1 %, 55.6 %, 53.7 % and 44.2 % respectively; as for sheep with Brucella infection, positive rate of the above mentioned 5 tests were 81.8 %. Conclusion Results showed that DAgS-EIA were not only specific and practicable but may use for Brucella specific antibodies detection in humans and various animals with only one conjugate of Brucella antigen with horseradish peroxidase.
【CateGory Index】: R446.6
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 7 Hits
1 LI Lan-yu,QIU Hai-yan,SHANG De-qiu,et al (Institute of Epidemiology and Microbiology,Chinese Academy preventive Medicine,Beijing,102206);A study on the identification diagnosis of infection about Brucella SPP and Yersinia enterocolitia(Ⅱ)[J];Chinese Journal of Control of Endemic Disenaces;2002-01
2 LIU Shu-ping,GUO Zhi-xi,WANG Ceng-liang,et al(Center for Disease Control and Prevention in Shanxi Province,TaiYuan 030013);Study on double antigens sandwich Enzymelinked Immunosorbent Assay for detection of Brucella specific antibodies in human and animals[J];Chinese Journal of Control of Endemic Disenaces;2002-04
3 ZHAO Hong-yan,LI Lan-yu,CUI Bu-yun,et al(National Institute for communicable Disease contral and Prevetion,Chinese center for Disease control and Prevention,Beijing 102206);Study on 5 serological tests with Brucella antigen for milk ring test(MRT) detection Brucella antibodies in human and animals Brucellosis[J];Chinese Journal of Control of Endemic Diseases;2006-04
4 ZHU Ming-dong,YANG Rong,HONG Lin-di,ZHONG Chun-mei,ZHAO Wen-yu.Zhejiang Academy of Medical Sciences,Hangzhou 310013,China;On-the-spot application of colloidal gold-immunochromatographic assay in various epidemic areas of brucellosis[J];Disease Surveillance;2008-05
5 LIU Xiao-he,HAN Wen-yu(Animal science and verterinery College of Jilin University,Changchun Jilin,130062,China);The Current Research on Brucellosis[J];Journal of Hebei Normal University of Science & Technology;2011-01
6 KOU Zeng-qiang,FENG Kai-jun,LI Zhong,et al.(Shandong Center for Disease Control and Prevention,Ji'nan,250014,Shandong,China);The Study on Evaluation of the Double Antigen Sandwich Enzyme Immunoassay for the Detection of Specific Antibodies in Brucellosis Cases[J];Preventive Medicine Tribune;2010-05
7 WANG Jia,XU Wei-min (Hangzhou Center for Disease Control and Prevention,Hangzhou 310006,China);Advanced on the study of serodiagnosis methods for brucellosis[J];Journal of Pathogen Biology;2008-02
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Lu Qifa, et al Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicate, Beijing;A STUDY ON SEROLOGICAL DIFFERENTIATION FOR BOTH ANTBODIES AGAINST BRUCELLA AND YERSINIA ENTEROCOLITICA SEROTYPE 0 : 9[J];Chinese Journal of Control of Endemic Disease;1995-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 XIE Zhi-xun, PANG Yao-shang, DENG Xian-wen, TANG Xiao-fei, LIU Jia-bo(Guangxi Veterinary Research Institute of Guangxi Zhuangzu Autonomous Region, Guangxi, Nanning 530001,China);Studies on Development of Multiplex RT-PCR for Simutaneous Detection of Three Kinds of Viruses in Penaeid Shrimp[J];Chinese Journal of Virology;2005-05
2 Tuerhong NUER1,GU Wen-xi1,HE Qian-ni1,Rehemujang2,LIU Li-ya1,ZHONG Qi1(1.Veterinary Research Institute,Xinjiang Academy of Animal Science,Urumqi,Xinjiang 830000,China;2 General Station of Animal Epidemic Prevention and Supervision of Xinjiang Uygur Autonomous Region,Urumqi,Xinjiang 830063,China);Study on New Technology for Rapid Diagnosis of Brucella abortus by DOT-ELISA[J];Endemic Diseases Bulletin;2008-01
3 $$$$Tang Liuying ,Shang Deqiu(Institute of Epidemiology and Microbiology,Chinese Academy of Preventive Medicine Beijing 102206);The Polymerase Chain Reaction(PCR )in Diagnosis of Brucella Infection[J];ENDEMIC DISEASES BULLETIN (CHINA);1994-03
4 Lu Qifa, et al Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicate, Beijing;A STUDY ON SEROLOGICAL DIFFERENTIATION FOR BOTH ANTBODIES AGAINST BRUCELLA AND YERSINIA ENTEROCOLITICA SEROTYPE 0 : 9[J];Chinese Journal of Control of Endemic Disease;1995-02
5 Tang Liuying,et al The Institute of Epidemiology and Microbiology , Chinese Academy of Preventive Medicine;DETECTION OF BRUCELLA USING MOIECUIAR TECHNIQUES (I)DETECTION OF BRUCELLA BY PCR[J];Chinese Journal of Control of Endemic Disease;1995-04
6 Tang Liuying ,et al Department of Epidemiolog y ,Microbiology of Chinese Preventive Medical Sciences;DETECTION OF BRUCELLA USING MOLECULAR TECHNIQUES : II DIAGNOSIS OF ANIMAL BRUCELLOSIS BY PCR[J];Chinese Journal of Control of Endemic Disease;1997-04
7 Shang Deqiu et al Institute of Epidemiology and Microbiology, Chinese Academy Preventive Medicine, Beijing;A STUDY ON THE NEW METHODS OF DIAGNOSIS FOR BRUCELLOSIS[J];Chinese Journal of Control of Endemic Disease;1999-03
8 Li Lanyu,Qiu HaiYin, Shang DaQiu (Institute of Epidemilogy and Microbiology, Chinese Academy Preventwe Medicine, Beijing, 102206);A study on the PCR about primers of 31KD_a protein gene for B.abortus[J];Chinese Journal of Control of Endemic Disenaces;2000-04
9 LI Lan-yu,QIU Hai-yan,SHANG De-qiu,et al (Institute of Epidemiology and Microbiology,Chinese Academy preventive Medicine,Beijing,102206);A study on the identification diagnosis of infection about Brucella SPP and Yersinia enterocolitia(Ⅱ)[J];Chinese Journal of Control of Endemic Disenaces;2002-01
10 LI Tie -feng,WANG Ji -qiu,GANG Sen-lin,et al(The First Institute of Endemic Disea se of Jilin Province,Baicheng 137000);A study of detecting Brucella DNA by PCR[J];Chinese Journal of Control of Endemic Disenaces;2002-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Hong-yan,LU Qi-fa (National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206);Experimental Study on double antibodies sandwich Enzymelinked Immurosorbent Assay for detection of Brucella antigens[J];Chinese Journal of Control of Endemic Disease;2004-03
2 ZHANG Shuang-zhai,ZHAO Dong,LIU Wei-hua ,et al(Shijiazhuang centre for disease control and prevention, Shijiazhuang 050011);A comparative analysis of SAT and MAT for brucellosis diagnosis[J];Chinese Journal of Control of Endemic Diseases;2008-03
3 MI Jing-chuan,ZHANG Qing-hua,YU Rui-ping,et al (Inner Mongolia Center of Endemic Disease Control and Research,Huhehot 010031);The epidemiological characteristics of human Brucellosis in Inner Mongolia[J];Chinese Journal of Control of Endemic Diseases;2010-01
4 TUERHONG Nu-er1,GU Wen-xi1,HE Qian-ni1,Rehemujiang2,BUHILIQIEMU Yiganbadi3,LIU Li-ya1(1.Veterinary Institute,Xinjiang Academy of Animal Sciences,Urumqi,Xinjiang,830000,China;2.Xinjiang Animal Epidemic Prevention And Monitoring Station,Urumqi,Xinjiang,830063,China;3.Livestock Bureau of Kashgar City,Kashgar,Xinjiang,844000,China);Advance in Brucellosis[J];Progress in Veterinary Medicine;2007-07
5 XING Jin1,WANG Jin-feng2,ZHAO Bao-hua1(1.College of Life Science, Hebei Normal University, Shijiazhuang,Hebei,050016,China;2.Health Authority, Board of Health, Luan County, Tangshan City,Hebei,063700,China);Progress on Brucellosis and Diagnostic Methods[J];Progress in Veterinary Medicine;2009-03
6 Chen Huiling,Ye Huifen,Yang Yinmei,Long Youmin.The First Municipal People's Hospital of Guangzhou,Guangzhou 510180,China;Analysis of 5 Cases Brucella in Blood Culture[J];Guangzhou Medical Journal;2007-04
7 ZHAO Feng-ju(Liaoning Center for Animal Epidemic Disease Control and Prevention,Shenyang 110161,China);Research Progress of Diagnosis Technique of Brucellosis[J];China Animal Husbandry & Veterinary Medicine;2011-08
8 Fu Lina Zhou Moju Fu Liwei;Investigation of Well Further Purification Brucella Vaccines[J];Beijing Agriculture;2011-S1
9 REN Ze-ping. Shanxi Provincial CDC,Taiyuan 030012,China;Analysis on the epidemic situation of brucellosis in Shanxi Province from 1996 to 2006[J];Disease Surveillance;2007-07
10 CHEN Wen-jing*, CUI Bu-yun, WU Mei-he, LI Lan-yu, LI Kai, ZHAO Hong-yan. *Hui Min District Sanitary and Anti-epidemic Station of Hu He Hao Te city, Hu He Hao Te 010030, China;Serological surveillance on brucellosis in both human and livestock[J];Disease Surveillance;2008-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved