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《Chinese Journal of Physical Medicine and Rehabilitation》 2004-01
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The effects of pulsed electromagnetic fields on the proliferation of bone marrow stem cells in mice

FANG Zhen-hua *, WU Hua, MA Wei-ming,ZHAO Wen-chun. *Departme nt of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China  
Objective To study the effect of pulsed el ectromagnetic fields (PEMFs) on the proliferation of bone mesenchymal stem cells in mice. Methods The bone marrow mesenchymal stem ce lls were obtained by using gradient centrifuge method, and then selected by the adhesive method. The third generation cells were irradiated by use of pulsed el ectromagnetic fields of 50Hz,sinusoid wave, at different intensities and for dif ferent time. The method of MTT was employed to evaluate the level of proliferati on. The parameters regarding the variation of the cell cycle were detected with the flow cytometry (FCM). Results The proliferation o f the bone mesenchymal stem cells in mice was greatly accelerated after 3 days o f irradiation by pulsed electromagnetic fields of 50Hz,sinusoid wave (twice dail y, 30 min each time ) at the intensities of 4mT,3mT,2mT and 1mT. The percentage of cells at the (S+G2/M) phase was increased significantly ( P 0.05). No abno rmal ploidy was found in every group. Conclusion Thre e days of irradiation (twice daily, 30 min each time) of pulsed electromagnetic fields of 50Hz,sinusoid wave at the intensities of 4mT, 3mT, 2mT and 1mT could i ncrease the proliferation of bone mesenchymal stem cells of mice in vitro.
【CateGory Index】: R329.2
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