Changes of Nitric Oxide Synthase in Hippocampus and Cerebellum of the Rat Following Exposure to Electromagnetic Pulse
Ding Guirong, Xie Xuejun, Zhang Liyan,et al.Department of Radiation Medicine, Fourth Military Medical University, 710032
Objective In order to explore the role of nitric oxide in the obstruction of learning and memory of the rat caused by exposing to electromagnetic pulse(EMP),the distribution of nitric oxide synthase(NOS) expression was studied in hippocampus and cerebellum of the rat following exposure to EMP. Methods SP immunohistochemical staining was employed to detect the distribution of NOS expression. Results The number of NOS positive neurons and the intensity of positive staining in hippocampus were decreased at 1.5 and 24h after exposure to EMP. At 48h,the number of NOS positive neurons reversed to control level but the intensity of positive staining was still lower.The expression of NOS in cerebellum had no obvious changes.Conclusion Decrease of NOS expression in hippocampus relates to the obstruction of learning and memory of the rat after exposure to EMP.