Experimental study on the damage to cells from fat removal and suction under negative pressures.
ZHANG Xinhe *, GAO Jianhua. *Department of Burn and Plastic Surgery, 174 Hospital of PLA, Xiamen 361003, China
Objective To study the injury percentage of fat cells from human bodies by three methods and four suctioned negative pressures. Methods Fat tissue was removed from lower abdomen by excision, aspiration and suction. A pair of sharp scissors was used to cut excisied adipose tissue into 3mm diameter fat particles. A 4 mm diameter pipet and four negative pressures (-25 kPa, -50 kPa, -76 kPa and -101 kPa) were used to suction fat tissue. The samples were fixed in 10 % buffered formalin, stained with hematoxylin eosin and Wilder's silver methods. The fat cells were observed under light microscope. Results The injury percentage of fat cells removed by three methods were (8.6± 1.8) %, (15.7±2.4) % and (38.4±4.2) % respectively;all of them had significant differences among them. The injury percents of fat cells suctioned under-25 kPa,-50 kPa, -76 kPa and -101 kPa were (26.3±2.5) %, (27.6± 2.6) %, (36.8±3.5) % and (68.9±7.4) % respectively. There were no significant differences between the first two but there were among other groups. Conclusions The injury extent of fat cells has significant differences among 3 methods, in which the excision is the least and the suction is the most serious. The injury extent of fat cells has no significant differences when the pressure is lower than -25 kPa. But when the pressure is higher than -50 kPa, the higher the pressure, the more serious the injury extent.
【CateGory Index】： R622