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《CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM》 1999-04
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Measurement of 5α reductase activity with cultured male genital skin fibroblast monolayer

WU Xueyan,SHI Yifan,DENG Jieying.Department of Endocrinology,Peking Union Medical College Hospital,Beijing,100730  
Objective To improve the diagnosis and treatment of sex differentiation disorder. Methods The 5α reductase activity measurement was established with the intact genital skin fibroblast monolayer. Results The inter and intra coefficients of variability were 6.4% and 8.9% respectively. After extraction and chromatography, the total recovery rate of 3H DHT was 91.5%. The value of 5α reductase activity in 8 normal males were (7.00±9.41)pmol·mg -1 ·h -1 (1.08~23.21pmol·mg -1 ·h -1 ). Conclusion The method of 5α reductase activity mesurement was stable and an evident variability was revealed among normal males.
【Key Words】: Pseudohermaphroditism 5α reductase
【CateGory Index】: R588
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【References】
Chinese Journal Full-text Database 1 Hits
1 WU Xue-yan,SHI Yi-fan,DENG Jie-ying(Department of Endocrinology,Peking Union Medical College Hospital,CAMS & PUMC,Beijing 100730,China);Androgen receptor binding assay with male genital skin fibroblast monolayer[J];Basic Medical Sciences and Clinics;2004-03
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Cui Yugui,Zhang Guiyuan,Tian Qunjie,et al.Department of Endocrinology, The First Affiliated Hospital, Nanjing Medical University, Nanjing, 210029.;APPLICATION OF METHODS FOR ASSAY OF 5α REDUCTASE ACTIVITIES AND ANDROGEN RECEPTOR IN CLINICAL PRACTICE[J];CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM;1997-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Lu Yan hua Pang Zheng zhi Wang Lei Zhao Yong sheng (College of Materials Science and Engineering ,Beijing University of Chemical Technology, Beijing 100029, China);Quantitative analysis of benzimidazole derivative[J];Journal of Beijing University of Chemical Technology;2003-06
2 Feng Yifan, Meng Qing, Li Weiseng (Guangdong College of Phmarcy, Guangzhou 510224);Determination of Ferulic Acid in Shen Rong Bai Feng Wan by HPLC[J];ACADEMIC JOURNAL OF GUANGDONG COLLEGE OF PHARMACY;1996-01
3 LAN Liu bo,DENG Yi feng,LIANG Nian ci,et al (Department of Biochemistry,Guangdong Medical College,Zhanjiang 524023);A new method for isolating and purifying the active compound 5F from Pteris semipinnate L.[J];Journal of Guangdong Medical College;2003-02
4 CHEN Cong-Jin HUANG Ke-Yinga WEI Long-Binb(Chemistry and Chemical Engineering College,Guangxi University,Nanning 530004,P.R.China)a(College of Material Science and Engineering,Central South University of Forestry and Technology,Zhuzhou,Hunan 412006,P.R.China)b(Guangxi Cadre School of Forestry,Nanning 530001,P.R.China);Advances of the Chromatography Methods in Determination of Flavoniods[J];Chinese Journal of Spectroscopy Laboratory;2007-05
5 CUI Yugui1, FU Guangbo2, ZHANG Guiyuan3, QIAN Lixin4, DI Fusong5. 1 Center of Clinical Reproductive Medicine, 4 Department of Urology, and 5 Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China; 2 Department of Urology, The Affiliated Huaian First People's Hospital of Nanjing Medical University, Huaian 214002, China; 3 National Research Institute for Family Planning, Beijing 100081, China Corresponding author: CUI Yugui, E-mail: cuiygnj@njmu.ed.com;Physiological Variation of Androgen Levels (T and DHT) in Target Tissues at Different Developmental Stages of Male[J];Journal of International Reproductive Health/Family Planning;2009-01
6 Lu Fuping, Lin Weitie, Yao Ruhua (Dept. of Bioengineering, South China University of'Technology GuangZhou 510641);Isolation and Extraction of Two Hydrophilic Antibiotic Substances[J];Industrial Microbiology;1996-04
7 WANG Zi-qiang,TANG Guang-shi*,LIU Shu-zhi,CHEN Xiao-lian,ZHANG Sheng-yuan (The Faculty of Science,Beijing University of Chemical Technology,Beijing 100029,China);Study on column chromatography for separation of C_(60) derivatives[J];Chemical Reagents;2005-12
8 DU Ying-xiang 1, LIU Wen-ying 2 (1. Department of Analytical Chemistry; 2. Department of Pharmaceutical Analysis, China Pharmaceutical University, Jiangsu Nanjing 210009, China);Determination of Amoxicillin and Metronidazole in Bijiaxilin Tablet by Dual-wavelength Thin-layer Scanning Technique[J];Huaxue Shijie (Chemical World);2001-05
9 ZHAO Xian ying,JI Wei gang,WANG Xiang zhi,QING Jun (Chemical Department of Third Military Medicial University,Chongqing 400038,China);Application of thin layer chromatography in the process of preparation of β-d-2-amino glucopyranose tetra acetyl ester[J];Chemical Research and Application;2002-03
10 PENG Yi-ming,JIANG Du-xiao,LIU Hui-qiong(Dept. of Chemistry, Jinan University, Guangzhou 510326, China);Determination of the content of lecithin with thin-layer chromatographic scanner[J];Journal of Jinan University(Natural Science & Medicine Edition);2002-05
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