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Measurement of 5α reductase activity with cultured male genital skin fibroblast monolayer

WU Xueyan,SHI Yifan,DENG Jieying.Department of Endocrinology,Peking Union Medical College Hospital,Beijing,100730  
Objective To improve the diagnosis and treatment of sex differentiation disorder. Methods The 5α reductase activity measurement was established with the intact genital skin fibroblast monolayer. Results The inter and intra coefficients of variability were 6.4% and 8.9% respectively. After extraction and chromatography, the total recovery rate of 3H DHT was 91.5%. The value of 5α reductase activity in 8 normal males were (7.00±9.41)pmol·mg -1 ·h -1 (1.08~23.21pmol·mg -1 ·h -1 ). Conclusion The method of 5α reductase activity mesurement was stable and an evident variability was revealed among normal males.
【CateGory Index】: R588
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