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《CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM》 1997-01
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APPLICATION OF METHODS FOR ASSAY OF 5α REDUCTASE ACTIVITIES AND ANDROGEN RECEPTOR IN CLINICAL PRACTICE

Cui Yugui,Zhang Guiyuan,Tian Qunjie,et al.Department of Endocrinology, The First Affiliated Hospital, Nanjing Medical University, Nanjing, 210029.  
Androgen receptor and 5α reductase play crucial roles in androgen action and metabolism. Their defect would cause the clinical male pseudohermaphroditism, defined as androgen insensitive syndrome and 5α reductase deficiency, respectively. We developed the methods for characterising the 5α reductase isozyme I and II, and for measuring ligand binding capacity of the androgen receptor in the genital skins of 40 normal men and 6 patients with male pseudohermaphroditism. Comparing with normal men of the same age ranges, 2 patients had much lower activities of 5α reductase isozyme II (2.6 and 0 DHT pmol/mg protein/30 min, respectively), but normal ligand binding capacity of androgen receptor. So they might be diagnosed as 5α reductase deficiency. Their diagnosis was further confirmed by the assay of 5α reductase activity dependent pH value of reaction system. In 3 patients with complete androgen insensitive syndrome, both cytosol and nuclear androgen receptor were defected and nuclear androgen receptor was only 7.27~15.74 fmol/mg protein and decreased 11.72~25.36 folds, although their 5α reductase was normal. So we suggest that 5α reductase activities and androgen receptor should be assayed in diagnosis and differential diagnosis of male pseudohermaphroditism.
【Key Words】: α Reductase Androgen Receptor Male Sex Differentiation Disorder.
【CateGory Index】: Q55
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【References】
Chinese Journal Full-text Database 3 Hits
1 CUI Yugui1, FU Guangbo2, ZHANG Guiyuan3, QIAN Lixin4, DI Fusong5. 1 Center of Clinical Reproductive Medicine, 4 Department of Urology, and 5 Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China; 2 Department of Urology, The Affiliated Huaian First People's Hospital of Nanjing Medical University, Huaian 214002, China; 3 National Research Institute for Family Planning, Beijing 100081, China Corresponding author: CUI Yugui, E-mail: cuiygnj@njmu.ed.com;Physiological Variation of Androgen Levels (T and DHT) in Target Tissues at Different Developmental Stages of Male[J];Journal of International Reproductive Health/Family Planning;2009-01
2 Yu-Gui CUI1, Gui-Yuan ZHANG2 (1. Department of Endocrinology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China; 2. National Research Institute of Family Planning, Beijing 100081, China);Steroid 5α-Reductase Activity, Androgen Level and Its Receptor in the Target Tissues of Male Fetuses[J];Acto andrologica Sinica;2001-04
3 WU Xueyan,SHI Yifan,DENG Jieying.Department of Endocrinology,Peking Union Medical College Hospital,Beijing,100730;Measurement of 5α reductase activity with cultured male genital skin fibroblast monolayer[J];CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM;1999-04
【Co-references】
Chinese Journal Full-text Database 4 Hits
1 Wang Suming, et al (Department of Biology);THE ESTABLISHMENT OF THE NORMAL HUMAN FIBROBLAST CELL LINE AND A STUDY OF ITS BIOLOGICAL CHARACTERIZATION[J];Academic Journal of Second Military Medical University;1986-01
2 CUI Yugui,ZHANG Guiyuan Department of Reproductive Endocrinology,National Research Institute for Family Planning,Beijing 100081;The steroid 5a-reductase isozyme activities in foreskin of Chinese adult men[J];JOURNAL OF REPRODUCTIVE MEDICINE;1995-01
3 CUI YuGui,ZHANG GuiYuan,LI Yian,YU Qi,He Fangfang,TIAN Qinjie,GE Qinsheng De partment of Reproductice Endocrinology,National Research Institute for Family Planning, Beijing 100081 *Peking Union Medical College Hospilal;Study on the cytosol and nuclear androgen receptor of normal male adults and patients with androgen insensitivity syndrome[J];JOURNAL OF REPRODUCTIVE MEDICINE;1995-04
4 Cui Yugui,Zhang Guiyuan.Department of Endocrinology,the First Affiliated Hospital of Nanjing Medical University,Nanjing,210029;Physiological variation of androgens and 5α reductase activities in male foreskin at different development stage[J];CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM;1998-05
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 WU Xue-yan,SHI Yi-fan,DENG Jie-ying(Department of Endocrinology,Peking Union Medical College Hospital,CAMS & PUMC,Beijing 100730,China);Androgen receptor binding assay with male genital skin fibroblast monolayer[J];Basic Medical Sciences and Clinics;2004-03
2 Hong Jun WANG 1 , Jia Ying ZHANG 2 , Su Huan LIU 1 , Hui ZHAO 1 , Zhong Shan WANG 1 (1 Department of Cell Biology, 2 the Second General Laboratory, School of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, China);Changes of 5 Alpha-reductase Type Ⅱ Activity in Sexual Gland of Diabetic Male Rats[J];Acto andrologica Sinica;2003-02
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