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《Chinese Journal of Internal Medicine》 2001-01
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Expression of Fas on monocytes and the level of plasma soluble FasL in patients with chronic renal failure

JIANG Jianping, HOU Fanfan, LIU Zhiqiang, et al Renal Division, Nanfang Hospital, Guangzhou 510515, China  
Objective The dysfunction of monocytes in uremic patients has been demonstrated to be associated with monocyte accelerated apoptosis The study was conducted toelucidate the mechanisms by which monocyte apoptosis was induced Methods seven non dialyzed chronic uremic patients and 18 hemodialysis (HD) patients were enrolled in the study Fifteen healthy volunteers were selected as controls The expression of Fas and Fas ligand (FasL) was examined by immunofluorescent staining and flow cytometer analysis The levels of soluble FasL (sFasL) in plasma were analyzed by enzyme linked immunosorbent assay (ELISA) The percentage of apoptotic cells in monocytes cultured in vitro with recombinant human FasL (rHu FasL) was quantitated by DNA content analysis after extraction of the degraded DNA Monocyte survival rate was analyzed by 5 diphenyl tetrazolium bromide (MTT) staining Results Expression of Fas on monocytes was significantly higher in uremic and HD patients than that in healthy subjects Higher levels of Fas expression were demonstrated on monocytes from HD as patients compared with those from non dialyzed patients There was no difference in monocyte Fas expression between pre and post dialysis session and between patients using polysulfone and cellulose dialyzer There was no detectable FasL expression on monocytes from both uremic patients and healthy controls Plasma sFasL could be detected in uremic and HD patients, but not in healthy controls There was no significant difference in levels of plasma sFasL between non dialyzed and HD patients, pre and post dialysis session, and patients treated with polysulfone and cellulose dialyzer When monocytes were cultured in vitro with rHu FasL for 2 hours, higher levels of apoptosis and lower survival rate were found in monocytes from HD patients as compared with those from healthy controls Conclusion The up regulated expression of functional Fas on monocytes and the presence of sFasL in plasma may contribute to the accelerated apoptosis of monocytes seen in uremic patients
【CateGory Index】: R692.5
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【References】
Chinese Journal Full-text Database 4 Hits
1 DING Lili, XIE Songye, CAI Yiting. Department of Clinical Laboratory, The First People’s Hospital, Shanghai Jiaotong University, Shanghai 200080, China;The clinical observation of serum soluble Fas and soluble Fas ligand levels in renal allograft rejection patients[J];检验医学;2005-01
2 YU Lixin1, MA JUNjie1, YANG Lijian2, LI YaNgqiu2, LIU Qifa3, XU Bing3 1Dept. of Kidney Transplantation, Nan Fang Hospital, Guangzhou, 510515; 2Dept. of Hematology, Jinan University, Guangzhou, 510632; 3Dept. of Hematology, Nan Fang Hospital, Guangzhou, 510515;Genescan analysis of clonality of TCR Vβ repertoire T cell in patients with maintenance hemodialysis[J];肾脏病与透析肾移植杂志;2004-01
3 JIN Jiansheng,ZHENG Xiangxiong,LIN Qingyou,et al Department of nephrology,Union Hospital,Fujian Medical University,Fuzhou (350001);Effect of Radix Astragali on sFasL and T Cell Subsets in Patients with Uremia[J];中国中西医结合肾病杂志;2002-03
4 CAI Bei, WANG Lan-lan, MI Xu-hua. Division of Clinical Immunology,Department of Laboratory Medicine of West China Hospital,Sichuan University,Chengdu 610041,China;Research on influences of haemodialvsis duration on immune functions of patients with uremia[J];检验医师杂志;2005-02
【Co-references】
Chinese Journal Full-text Database 4 Hits
1 JIANG Jianping, HOU Fanfan, LIU Zhiqiang, et al Renal Division, Nanfang Hospital, Guangzhou 510515, China;Expression of Fas on monocytes and the level of plasma soluble FasL in patients with chronic renal failure[J];中华内科杂志;2001-01
2 Xia Chengqing, Zhao Hongying, Tang Xiaonai Department of Pathology, Beijing Red Cross Chaoyang Hospital(Beijing,100020);THE INCREASE OF RENAL FAS AND FAS L EXPRESSION IS ASSOCIATED WITH RENAL ALLOGRAFT REJECTION[J];肾脏病与透析肾移植杂志;1998-04
3 Li Yangqiu, Wang Mingchun.Departmert of Hematology, Medical College, Jinan University, Guangzhou 510632Wu Youhua. Departmert of Biology & Chemistry, Shantou College of Education, Shantou 515041;T CELL CLONALITY IDETIFICATION USING GENESCAN BY CDR3 SIZE OF T CELL RECEPTOR Vβ GENE[J];中国免疫学杂志;1998-01
4 JiangJianping,ZhangXun,HouFanfan.DepartmentofNephrology,NanfangHospital,Guangzhou510515;TheefectofrHuEPOonthecytokinesecretionofmononuclearcelsinmaintainedhemodialysispatients[J];中华肾脏病杂志;1996-03
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 LI Yong-xin,YIN Qing-song,WANG Xiao-jiao,LI Qian-Yu,WEI Xu-dong.Department of Nephrology,First People's Hospital of Zhengzhou,Zhengzhou 450008,China;Measurement of recent thymic output function in uremia patients who underwent maintenance hemodialysis[J];中国实用内科杂志;2012-10
2 LIU Yi,LIN Fan,ZHENG Chenfei,et al The First Affiliated Hospital, Wenzhou Medical College,Wenzhou (325000);Renal Protection of Mongelian Milkvetch Root on Radiocontrast Acute Renal Failure in Diabetic Rats[J];中国中西医结合肾病杂志;2003-10
3 ;Clinical observation of treating different hematuria with two Chinese traditional therapy[J];中华实用中西医杂志;2005-13
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