Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《CHINESE JOURNAL OF INTERNAL MEDICINE》 1999-06
Add to Favorite Get Latest Update

Clearance of intrahepatic hepatitis B virus by a nonimmune mechanism

ZHU Youfu, LUO Kangxian, WANG Jinyu. Department of Infectious Diseases, Nanfang Hospital, Guangzhou 510515  
Objective The clearance of intrahepatic hepatitis B virus(HBV) is mainly by the cytotoxicity of immune cells with ways including CD95ligand/CD95 induction. We found that the liver cells do express CD95 ligand(CD95L), a cytotoxic cellsurface molecule. This paper studied the mechanism and implications of the hepatocytes themselves to clear the virus. Methods The HepG2 cells with induced CD95L and the HepG2.2.15 cells with constitutive CD95 expression were coincubated. The apoptotic cells were examined under fluorescene microscope and with flow cytometry. Results In chronic hepatitis B intrahepatic expressions of CD95L and/or CD95 were detected at both mRNA and protein level. Methotrexate induced CD95L expression of HepG2 cell. The HepG2 cells with CD95L could kill the HepG2.2.15 cells with CD95 molecule with apoptotic rates of 16.5% at 24h and 43.0% at 48h. This apoptosis effect could be blocked by using antiCD95L antibody. Conclusion The hepatocyte might induce apoptosis mutually and clear the virus themselves with a nonimmune mechanism.
【CateGory Index】: R735.7
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 1 Hits
1 hu Youfu, Luo Kangxian He Haitang, et al, Nanfang Hospital, Guangzhou 510515.;HEPATOCYTE FAS EXPRESSION AND APOPTOSIS IN HEPATITlS B VIRUS RELATED CHRONIC UVER DIS EASES 2[J];CHINESE JOURNAL OF HEPATOLOGY;1997-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 XIE Qing xiang, WANG Hong, MIAO You reng, et al. The Department of Urology,The 175th Hospital of PLA,Zhangzhou 363000,P.R. China;The expressions of Fas and FasL in 72 cases with transitional cell cancer of bladder[J];CHINESE JOURNAL OF CANCER;2000-02
2 WU Gang, YAN Ge, WU Guo-yi,et al. (Institute for Viral Hepatitis, Chongqing University of Medical Sciences,Chongqing 400010,China);The study of N-acetylcysteine-restrained apoptosis of human hepatocyte in vitro[J];Chongqing Medical Journal;2005-01
3 YU Hong, CAO Zhi-chen, GENG Jian-ying, HE Zhan-guo, WANG Zheng-min, SUN Xiao-yun, WANG Zhong-huaDepartment of Hepatopathy, Bethune International Peace Hospital of PLA, Shijiazhuang 050082, China;Effect of interferon alpha on liver fibrosis in patients with chronic hepatitis B[J];Journal of First Military Medical University;2005-11
4 LAI Zhi-wei 1,WANG Qiao-zhen 1,WEN Bo-xian 2 (1 Qingyan Peoples Hospital, Qingyuan 511500,China;2 Guangzhou No 6 Peoples Hospital,Guangzhou 510655,China);Research in Curative Effects on 40 Cases with Viral Hepatitis B by Hepatic Growth Factor[J];TRACE ELEMENTS SCIENCE;2000-05
5 CHEN Wen-gui,XIE Hai-tao,LIU Yong-he (Xiangya School of Central South University,Changsha,Hunan 410013,China);The Effects of the Expression of HBV X Gene on Apoptosis in HepG2 Cell[J];Journal of Hunan Normal University(Medical Sciences);2006-01
6 ZHU Wanqin,GU Wei,DONG Xin.Department of Emergency Medicine,First Clinical College,China Medical University,Shenyang 110001,China;Acetaminophen-induced apoptosis of hepatocyte in rats[J];Journal of Emergency Medicine;2003-06
7 Guo Linlang; Guo Ying; Cao Changan(Zhujiang Hospital ,First Military University, Guangzhou, 510282);Expression of protease ICH-1 in chronic hepatitis B[J];CHINESE JOURNAL OF CLINICAL HEPATOLOGY;1999-03
8 WANG Jun xue,WANG Guo jun,CAI Xiong(Department of Infectious Diseases,ChangZheng Hospital,The Second Military Medical University,Shanghai 200003);The effects of oxymatrine on apoptosis induced by TNF-α in human liver cell line L02[J];Chinese Journal of Clinical Hepatology;2001-04
9 SHENG Lei, ZHU Li-min, ZHANG Wan-zeng, et al. (Tianjin Infectious Disease Hospital, TianJin 300192,China);The Study of Relationship Between FAS Antigen Expression and HCVRNA Level of Patiens with Chronic Hepatitis C[J];Chinese Journal of Clinical Hepatology;2004-05
10 YU - Hong, GENG Jian - ying, SUN Xiao - yun, et al.( Bethune Internat ional Peace Hospital of PLA Hepatology 050082);The clinic study of interfern alpha on liver flbrosis of patients with chronic hepatitis B[J];Chinese Journal of Clinical Hepatology;2005-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved