Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Archives of General Surgery(Electronic Edition)》 2012-03
Add to Favorite Get Latest Update

Silencing expression of SPHK1 gene induces the apoptosis of human gastric cancer SGC-7901 cells in vitro

JIAN Wei-hua, ZHANG Luan-jing , LIU Long-shan, WU Zhao-feng, LAI Yue-yuan, WENMin-jie, CAI Wei-shan, ZHANG Long-juan, XIA Jin-tang. *Department of Surgery, The First Municipal People’s Hospital of Guangzhou, Guangzhou Medical College , Laboratory of Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China  
Objective To investigate the effect of the siRNA targeting SPHK1 gene on the apoptosis of SGC-7901 cells of human gastric cancer in vitro. Methods Specific small interfering RNA (siRNA)targeting SPHK1 gene and control siRNA were chemically synthesized and transfected into SGC-7901 cells respectively. The expressions of SPHK1, Bcl-2 and Bax proteins were tested by Western blotting. And the apoptosis of SGC-7901 cells was tested by Flow Cytometry. Results In SPHK1 siRNA treated SGC-7901 cells, the protein expression of SPHK1 was significantly decreased (P 0.01). Compared with the control group, Bcl-2 protein level was reduced by 55% in the SPHK1 siRNA transfected group by 55% (P 0.01), with no significant difference for the expression of Bax protein while the ratio of Bcl-2/Bax protein expression was reduced by 54% (P 0.05). The number of the early apoptosis cells for the SPHK1 siRNA transfected group was significantly more than that for the control siRNA transfected group(P 0.01) but with no significant difference for the number of late apoptosis cells. Conclusions SPHK1 siRNA was able to specifically knock down the expression of SPHK1 and induce cell apoptosis by regulation the Bcl-2 pathway.
【Fund】: 广州市医药卫生科技计划项目(201102A212012)
【CateGory Index】: R735.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved