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《Chinese Journal of Organ Transplantation》 2003-05
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The role of mycophenolate acid on HBV replication in vitro

WU Jian,XIE Hai-yang,JIANG Guo-ping,et al. Department of Hepatobiliary Surgery,The First Affiliated Hospital of Medical College, Zhejiang University,Hangzhou 310003, China  
Objective To study the effect of mycophenolate acid(MPA)on HBV replication and viral protein synthesis in vitro. Methods The 2.2.15 cells were treated with different concentrations of MPA(1-50?mg/L) for 12 days. HBsAg and HBeAg in supernatant were detected by ELISA and intracellular HBV DNA was analyzed by slot blot hybridization. Results MPA could suppress the expression of HBsAg and HBeAg. The higher concentration induced the lower expression of HBsAg and HBeAg. The suppression rate of HBsAg and HBeAg by MPA at the concentration of 50?mg/L was 24.05?% and 30.20?% respectively. The effect of MPA on HBV DNA was similar in HBsAg and HBeAg. HBV DNA was only 49?% of the controls at the concentration of 50?mg/L . Conclusion MPA can suppress the expression of HBsAg and HBeAg and replication of HBV DNA in the 2.2.15 cells in a dose-dependent manner.
【Key Words】: Mycophenolate acid Hepatitis B virus Antigens Cell line
【CateGory Index】: R965
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【References】
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【Co-citations】
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1 ZENG Xiao Li1, TU Zhi Guang2 1.Clinical Laboratory of Anzhen Hospital,Capital University of Medical Sciences, Beijing,100029, P.R.China 2.Department of Clinical Biochemistry,Chongqing University of Medical Sciences, Chongqing,400016, P.R.China;Induction of Differentiation by Ginsenoside Rh_2 in Hepatocarcinoma Cell SMMC-7721[J];Chinese Journal of Cancer;2004-08
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3 ZHANG JieLIAO Ya-ping,WU Ling-zhi,KONG Ling-ping,ZHEN Min,WANG Hua-qiao.Department of Anatomy,Preclinical College,Guangdong University of Pharmacy,Guangzhou 510006 China;Influence on human embryonic cortex neuron survival and apoptosis after transfected the hTERT gene[J];Anatomy Research;2008-04
4 LI Hong-yan,ZHAO Yu,ZHANG Wei,ZHANG Hui,WU Hai-ri,ZHAO Da-qing(Center for New Medicine Research,Changchun University of Traditional Chinese Medicine,Changchun 130117,China);Effect of Ginseng Hydrophilic Protein on Proliferation of Vero,L_(929) and 2BS Cell Lines[J];Journal of Jilin Agricultural University;2008-05
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6 Lin Zhang,Xiaofeng Pang (School of Life Science and Technology,University of Electronic Science and Technology of China Chengdu 610054,China);Effects of NanoTiO_2 on Proliferation of cell And Its Safety[J];Life Science Instruments;2007-07
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9 LI Hong-yan,ZHANG Wei,ZHANG Hui,WU Hai-ri,ZHAO Yu,ZHAO Da-qing* (Center for New Medicine Research of Changchun University of Traditional Chinese Medicine. Changchun 130117,China);Study of the Effect of Ginseng Hydrophilic Protein on the Proliferation of Vero Cells[J];Special Wild Economic Animal and Plant Research;2007-01
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【Co-references】
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1 WANG Yi-bin, YU Li-xin, DENG Wen-feng, MIAO Yun, YE Jun-sheng Center of Kidney Transplantation, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;Application of mycophenolate mofetil in hepatitis C patients after kidney transplantation[J];Journal of Southern Medical University;2006-08
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