Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Burns》 2001-04
Add to Favorite Get Latest Update

Bioactivity and test grafting of acellular dermal matrix containing fibroblasts

XJAO Shichu,XIA Zhaofan, YANG Jun, et al. Department of Burn, The Second Military Medical University, Shanghai, 200433 P. R. China  
Objective To investigate the bioactivity of acellular dermal matrix with fibroblasts and its role as dermal skeleton. Methods Human fibroblasts( HFs) were planted onto the surface of acellular der- mal matrix ( ADM) to form living dermal substitute. The 1L - 6, IL - 8 and TGF contents in the supernatant of the culture of HF - ADM were determined with ELISA method, and the secretion of hyaluronic acid and laminin from extracellular matrix was measured with RIA method. The speed of vascularization and the wound contracture rate were observed after the dermal substitute was grafted on the full skin loss wound of Balb/c - nu mice (nude mice). Results HFs grew very well after being planted onto ADM so as to form a single layer of celluar membrane. Many kinds of eytokines and extracelluar matrix components were secreted. Com- pared with simple acellular dermal grafting, the vascularization was accelerated, and the wound contracture rate decreased, after the living dermal substitute being grafted on the wound. Conclusion The ADM see- ded with HFs exhibited excellent bioactivity and might be an optimal dermal substitute.
【CateGory Index】: R644
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 WANG Hai-lun1,2,Lü Xian2,YU Chun-yan1,FENG Ya-qin1,FENG Feng1,ZHANG Yong-jie3,HUANG Sha3,DENG Tian-zheng3, ZHANG Rui-hong3,JIN Yan3 (1. Department of Dermatology,Tangdu Hospital, The Fourth Military Medical University,Xi' an 710038,Shanxi,China;2.93942 Unit Hospital of PLA, Xianyang 710012,Shanxi,China;3. Tissue Engineering Center,The Fourth Military Medical University,Xi'an 710032,Shanxi,China);Construction and characteristic study of tissue engineering granule skin[J];Biomedical Engineering and Clinical Medicine;2008-03
2 XIAO Shichu, YANG Jun, XIA Zhaofan, et al. Burn Center, Changhai Hospital,Second Military Medical University,Shanghai 200433, China;Preparation and implantation of a new substitute of acellular dermal matrix[J];Shanghai Medical Journal;2003-08
3 ZHANG Mingle, HUANG Xianghua, LI Yachai,et al (The Second Hospital of Hebei Medical University, Shijiazhuang Hebei 050000,China);Primary Experimental Study on Tissue Engineering Vagina Constructed by Mouse Vaginal Epithelial Cells and Porcine Acellular Dermal Matrix (PADM)-Fibrin Gel[J];Journal of Practical Obstetrics and Gynecology;2012-08
4 ZHANG Mingle,HUANG Xianghua,LI Yachai,et al(The Second Hospital of Hebei Medical University,Shijiazhuang Hebei 050000,China);Comparison between Vaginal Epithelial Cells Seeded and Unseeded Porcine Acellular Dermal Matrix for Vagina Reconstruction in Mice[J];Journal of Practical Obstetrics and Gynecology;2013-04
5 Hu Kuikui~1,Dai Yucheng~2,Yuan Jingdong~1(1.Center of Plastic,Laser and Cosmetic Surgery,the Second Affiliated Hospital ofJiangxi Medical College,Nanchang 330006;2.Key Laboratory of Molecular Medicineof Jiangxi Province,Nanchang 330006,China);An experimental study on the preparation of artificial dermis[J];Journal of Xi'an Jiaotong University(Medical Sciences);2006-02
6 XIAO Shi-chu,XIA Zhao-fan,YANG Jun,et a l.Changhai Hospital,Second Milita ry Medical University,Shanghai 200433,China;Reconstruction and transplantation of mixed culture skin substitute comprising xenogeneic and autologous keratinocytes[J];Modern Rehabilitation;2002-22
7 Chen Junyin1,Wei Hong2 1.Department of Pathology,Hospital 324 of PLA,Chongqing,400020,China;2.Basic Medical College,Third Military Medical University,Chongqing,400038,China;Study of transplantation of xeno-acellular dermal matrix in rats[J];Medical Journal of National Defending Forces in Southwest China;2010-07
8 NIU Zhen-hai,XUE Bao-sheng△,SUN Shu,TANG Ming-rui (Department of Burns,The First Affiliated Hospital,China Medical University,Shenyang 110001,China);Effect of topical application of low-dose insulin on revascularization process in acellular dermal matrix graft[J];Journal of China Medical University;2007-04
9 SU Qing-he,YANG Min-jie, ZHOU Hong-mei.Department of Burns, The Third People's Hospital, Wuxi 214041, Jiangsu Province, P.R.China;Repair of murine full skin loss with composite skin of collagen scaffold containing living cells[J];Chinese Journal of Burns;2003-06
10 XIAO Shi-chu, XIA Zhao-fan, YANG Jun, LIU Zhi-guo, BEN Dao-feng, YU Wei-rong, YANG Yong, ZHU Shi-hui. Department of Burn Surgery, Changhai Hospital, The Second Military Medical University, Shanghai 200433, P.R. China;Expression of some active peptides and extracellular matrix in the in vitro cultured tissue engineering skin[J];Chinese Journal of Burns;2003-S1
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Chen 1,LI Shu-ping 1,LI Tian-duo 1,FENG Jian-yan 2,GAO Fu-tang 1(1.Light Chemical and Environmental Eng School, Shandong Institute of Light Industry, Jinan 250100, China; 2.Leather Engineering Dept, Northwest Institute of Light Industry, Xianya;Study on Mantle Micro Structure of National Pigskin[J];Leather Chemicals;2002-02
2 Liu Dewu;Su Ziyi; Cao Yong, et al. Burn Centre, (The First Affiliated Hospital of Jiangxi Medical College,Nanchang 330006);The roles of several growth factors iu serum-free culture of human epidermal cells in vitro[J];CHINESE GENERAL PRACTICE;1999-03
3 ZHANG Li-qing, WANG Lei, WANG Xu-dong (School of Environmental & Municipal Engineering , Xi'an University of Architecture and Technology, Xi'an 710055, China );INFLUENCE OF MEMBRANE STRUCTURE AND CHEMICAL CHARACTERISTICS ON SEPARATION AND FOULING OF NANOFILTRATION MEMBRANES[J];Technology of Water Treatment;2009-01
4 Ye Huazhi et al(Sichuan Agricultural University, Yaan, Sichuan, China);APPLICAFION OF DOUBLE STRANDED RNA ANALYSIS TO DIAGNOSIS OF APPLE LATENT VIRUSES[J];Journal of Sichuan Agricultural University;1991-02
5 HUANG Su ping (Jiangsu Province Yancheng Textile Industry School,Yancheng 224005,China);The Applications of Silk Fibroin Membrane on Man-made Skin[J];Shandong Textile Science & Technology;2003-06
6 DONG Zhi-ning,SUN Fen-yong,PAN Qiu-hui,HONG An(Biotechnological Institute of Jinan University, Guangzhou 510632 China);The Study on Transfection of rhbFGF into Human Skin Fibroblast[J];Journal of Biomedical Engineering Research;2004-01
7 ZHANG Ai-jun1,SONG Guo-dong1,CAO Cheng-bo2,WANG Yun-shan3,BIAN Dong-hui1(1.Department of Burn and Plastic Surgery,Jinan Central Hospital,School of Clinical Medicine,Shandong University,Jinan 250013,Shandong,China;2.School of Chemistry and Chemical Engineering,Shandong University,Jinan 250100,Shandong,China;3.Central Laboratory,Jinan Central Hospital,School of Clinical Medicine,Shandong University,Jinan 250013,Shandong,China);An experimental study on the attachment and growth of human skin fibroblast on a porcine collagenic dermal matrix in vitro[J];Journal of Shandong University(Health Sciences);2007-07
8 Zuo Jinhua,Xu Huiying,Yang Youcheng,et al;Experimental study of the effect of Triton X-100 on the preparation of ADM[J];Shandong Medical Journal;2005-11
9 XIANG Jim, LIU Ying-kai, LU Shu-Hang, et al ( Department of Burn, Burn Institute of Shanghai, Ruijin Hospital, SSMU, Shanghai 200025, China);The Preparation of the Rabbit Acellular Dermal Matrix[J];Acta Universitatis Medicinalis Secondae Shanghai;2003-03
10 YANG Jun, YANG Guang-hui, LIU Wei, CUI Lei.CAO Yi-lin, QIAN Yun-liang (Department of Plastic and Reconstructive Surgery, The Ninth People's Hospital, Shanghai Tissue Engineeing Reseach and Development Center, Shanghai Second Medical University, Shanghai 200235 , China);Construction and clinical application of tissue engineered epidermal membrane in treatment of hypertrophic scar[J];Acta Universitatis Medicinalis Secondae Shanghai;2004-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 Zuo Jinhua,Xu Huiying,Yang Youcheng,et al;Experimental study of the effect of Triton X-100 on the preparation of ADM[J];Shandong Medical Journal;2005-11
2 WANG Hai-lun1,2,Lü Xian2,YU Chun-yan1,FENG Ya-qin1,FENG Feng1,ZHANG Yong-jie3,HUANG Sha3,DENG Tian-zheng3, ZHANG Rui-hong3,JIN Yan3 (1. Department of Dermatology,Tangdu Hospital, The Fourth Military Medical University,Xi' an 710038,Shanxi,China;2.93942 Unit Hospital of PLA, Xianyang 710012,Shanxi,China;3. Tissue Engineering Center,The Fourth Military Medical University,Xi'an 710032,Shanxi,China);Construction and characteristic study of tissue engineering granule skin[J];Biomedical Engineering and Clinical Medicine;2008-03
3 LI Hua,JI tong,XU Li-qun,HU Yong-jie,LI Si-yi, ZHANG Chen-ping. (Department of Oral and Maxillofacial Surgery, School of Stomatology; Ninth People's Hospital,School of Medicine, Shanghai Jiao Tong University. Shanghai 200011, China);Study on the osteogenesis ability of co-culturing bone marrow stromal cells (BMSCs) and small intestinal submucosa[J];Shanghai Journal of Stomatology;2006-02
4 CHEN Can,WANG Zhi-jun,LIU Wei,et al.(Department of Plastic Surgery,Xinhua Hospital Affiliated to Dalian University,Dalian 116021,China);The comparison of three different methods that affect the infiltration of porcine acellular dermal matrix[J];Chinese Journal of Aesthetic and Plastic Surgery;2008-04
5 Liu Xuexu 1 Wang Kunyu 2 Ding Yunping 3 He Xueling 1 Wang Bi 2 1(Laboratory Animal Center of Sichuan University, Chengdu 610041,China) 2(Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education,Chengdu 601165,China) 3(West China College of Stomayology, Sichuan University, Chengdu 610041,China);Restoring the Skin from Traumatism by Means of Collagen-konjac Glucomannan-chondroitin Sulfate Blend Film[J];Journal of Biomedical Engineering;2005-05
6 CAO Jian-hong1,HOU Ruan-ling2,MA Xiu-feng1,et al.1.Department of Burn,The 91th Central Hospital of the PLA,Jiaozuo Henan 454003,China;2 Department of Physiology and Neurobiology,Xinxiang Medical College,Xinxiang Henan 453003,China;The short and long term efficacy of autologous skin and fat particles mixed transplantation for treating joint deep burns[J];Journal of Clinical and Experimental Medicine;2012-24
7 ZHANG Mingle,HUANG Xianghua,LI Yachai,et al(The Second Hospital of Hebei Medical University,Shijiazhuang Hebei 050000,China);Comparison between Vaginal Epithelial Cells Seeded and Unseeded Porcine Acellular Dermal Matrix for Vagina Reconstruction in Mice[J];Journal of Practical Obstetrics and Gynecology;2013-04
8 ZHANG Zhi-qiang;HUANG Xiang-hua;ZHAO Lin-yuan;The Second Hospital of HeBei Medical University;;The Effects of Microenvironment on Cells and The Application of Bionics in Tissue Engineering Scaffolds[J];China Biotechnology;2014-04
9 ZHANG Shi-wu,CHEN Li,LIU Ya-min(Department of Pathology, Me dical College of Chinese People′s Armed Police Force, Tianjin 300162,China);Tri-dimension culture and morphologic study of malignant melanoma cell line[J];Acta Academiae Medicinae Cpapf;2004-02
10 LIU Xin-hua1,3, DAN Nian-hua1,3*, JU Hai-yan1,3, LI Zheng-jun1,2, DAN Wei-hua1,2,3 (1.The Biomass and Leather Engineering Department, Sichuan University, Chengdu 610065, China; 2. Suzhou Institute of Sichuan University, Suzhou 215000, China; 3.The Center of Biomedicine Engineering of Sichuan University, Chengdu 610065,China);Research Progress on Collagen-based Composite Medical Fiber Tissue Engineering Scaffold Material[J];West Leather;2012-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved