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The Effects of Sophora Flavescens Alkaloids (SFA) on Coxsackie Virus B3(CVB3)in Vitro and a Preliminary Study of Its Mechanism

Chen Fuxiang; Liu Jingxing; Lu Deyuan(Department of Microbiology, Shanghai Second Medical University, 200025)  
Sophora flavescens alkaloids (SFA) was extracted from sophora flavescens by acid percolation and chloroform extraction.The effects of SFA on Coxsackievirus B3 (CVB3) replication and capsid proteins were investigated in HeLa cell culture by SDS-PAGE and Western-blot. The results showed that SFA inhibited the CVB3 replication in vitro dose-dependently. Adding SFA before and after CVB3 was absorbed in and infected Hela cells. the similar inhibitory effects were observed. This suggested that both the absorption and penetration of CBV3 were unaffected by SFA and it could enter the cells and inhibitthe virus replication. Although the target of SFA activity remained to be elucidated, the Western-blot analysis of CVB3capsid showed that the biosynthesis of viral protein might be the site of inhibition.
【CateGory Index】: R373
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2 PANG Ji,GUO Jin-peng,JIN Min,SHEN Zhi-qiang,WANG Xin-wei,QIU Zhi-gang,LI Jun-wen(Institute of Hygiene and Environmental Medicine,Academy of Military Medical Sciences,Tianjin 300050,China);ANTI-ENTEROVIRUS EFFECTS OF AQUEOUS EXTRACT FROM SPATHOLOBUS SUBERECTUS DUNN IN VITRO AND IN VIVO[J];解放军预防医学杂志;2009-04
3 SONG Hui-wen, WANG Lin.Department of Cardiovasology, Baoan People's Hospital Affiliated to Southern Medical University, Shenzhen 518110, China;Effects of oxymatrine on the expression of intercellular adhesion molecule-1 in experimental left atrial fibrosis in rabbits with atrial fibrillation[J];实用医学杂志;2009-12
4 LEI Xiang(Wuhan University of Science and Technology,Zhongnan Branch,Wuhan 430223,China)CHEN Ke-li,CHEN Gang(Hubei College of Traditional Chinese Medicine,Wuhan 430064,China)YANG Zhan-qiu(Research Institute of Virus,Medical College of Wuhan University,Wuhan 430071,China);Experimental Study on the Antiviral Action of Emodin in Combination with Other Chinese Active Medicine Components on Coxsackie Virus B_3 Replication[J];中国药房;2009-03
5 WANG Hong-cheng,JIANG Xin-dao(Qingdao Municipal Hospital,Qingdao 266011);Progress on pharmacology of oxymatrine[J];齐鲁药事;2008-02
6 Jiang Mei,Yin Shusheng,Jian Qiang,et al;Effect of Oxymatrine in treatment of chronic hepatitis B[J];中国疗养医学;2007-10
7 CHEN Ling1,LUO Kai2,WU Yun1,YAN Fu-hua2.(1.Department of Stomatology,First Affiliated Hospital of Fujian Medical University,Fuzhou 350004,China; 2.Department of Stomatology,Affiliated Hospital of Stomatology,Fujian Medical University,Fuzhou 350002,China);Study progress of Oxymatrine about its Anti Inflammatory Action[J];医学综述;2007-15
8 Yu Guiqin1,Zhou Xiaohui2,Shi Xiaohua1,et al.1.The Second Affiliated Hospital of Shantou University,Shantou,Guangdong 515065;2.The First Affiliated Hopsital of Shantou University,Shantou,Guangdong 515065,China;Preliminary stuty on therapeutic effect of oxymatrine in treating patients with chronic hepatitis C[J];四川医学;2007-06
9 GUO Jin-peng,PANG Ji,WANG Xin-wei,et al.(Disease Prevention and Control Institute of the Chinese People's Liberation Army,Beijing 100071,China);Anti-enterovirus Effects of Aqueous Extracts From Caulis Spatholobi in Vitro[J];实用预防医学;2007-02
Chinese Journal Full-text Database 10 Hits
1 GAO Hong-quan,LIV Yue-guang,WANG Yu.Mudanjiang Medical College,Mudanjiang 157011 China;Oxymatrine induces apoptosis in ovarian cancer cell line HO8910[J];解剖学研究;2007-03
2 KONG Xiu-Yan, JIN Yu, LI Jin-Yu Department of Pediatrics, First Hospital, Lanzhou University, Lanzhou 730000, China;Experimental study of oxymatrine in preventing glomerulosclerosis in rats with adriamycin-induced nephropathy[J];第四军医大学学报;2006-23
3 YU Zhi-hua,FENG Yi-bai(Institute of Cardiology,Union Hospital,Tongji Medical College,Huazhong University of Science & Technology,Wuhan 430022,China);Protective Effect of Oxymatrine on Myocardial Ischemic Reperfusion Injury in Rats[J];中国药学杂志;2006-04
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5 ZHOU Ai Ling 1,2 , LUO Lin 1, MAO Jia Hui 2, ZHU Yan 2 1 Department of Pharmacology, 2 Department of Pathophysiology, Medical College of Nantong University, Nantong 226001, Jiangsu, China;Effects of kwoninone on experimental liver fibrosis and expression of matrix metalloproteinase-2 in rats[J];中国临床药理学与治疗学;2004-10
6 Zhang Fengling,Tang Yong,Zhang Jingmei Zhengzhou Yonghe Pharmaceutical Corp.Ltd.;Advance in Research into Mechanism and Pharmaceutics of Matrine and Kurarinone[J];河南中医学院学报;2004-03
7 GUO Xin1, GAO Xiang-dong1, YANG Xiao-bing2(1.School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China;2. School of Life Sciences, Nanjing University, Nanjing 210093, China);Determination of content glucuronic acid and neutral sugar of acidic polysaccharide[J];中国生化药物杂志;2004-02
8 HUANG Xiu mei 1,2 , LI Bo 1 (1.National Institute of the Control of Pharmaceutical and Biological Products, Beijing 100050, China; 2.Qinghai Province Institute for Drug Control, Xining 810000, China);Effect of oxymatrine on TNFα, IL-6 and IL-8 in mice[J];中成药;2003-11
9 HANG Xiu mei 1, LI Bo 2 (1. Qinghai Provincial Institute of Sanitation Supervision, Xining 810000, China;2. National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China);Effects of six alkaloids of Sophora Alopecuroides on LTC_4 and LTB_4[J];中成药;2003-10
10 LIU Mei, LIU Xue-ying, CHENG Jian-feng (TangDu Hospital, the Forth Military University, Xi'an 710038, Shaanxi, Chi na);Advance in the Pharmacological Research on Matrine[J];中国中药杂志;2003-09
【Secondary References】
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1 LI Hua-li;WANG Xiao-long;YE Xiao-ya;Pingdingshan Food and Drug Inspection Institute;;Simultaneous determination of 6 alkaloid constitutents in Kushen dispensing granule by HPLC-DAD[J];中南药学;2018-05
2 JIA Wei;MAO Shumin;ZHANG Panpan;YAN Guangling;JIN Juan;LIU Yuhong;Shandong University of Traditional Chinese Medicine;Feixian Food and Drug Administration;;Study on Antiviral Effect of Lonicera Japonica Thumb Polysaccharide in Vivo[J];辽宁中医药大学学报;2018-06
3 CHEN Jun-xian;LI Zuo-xiao;Department of Neurology,Leshan Hospital of Traditional Chinese Medicine;Department of Neurology,Affiliated Hospital of Southwest Medical University;;Intervention effect and its mechanism of oxymatrine on rats with experimental autoimmune encephalomyelitis[J];广西医学;2018-03
4 JI Jian-bin;ZHOU Zheng-hua;LI Chong;WU Zheng-qing;DING Wei;Department of Spleen and Stomach Disease,First Teaching Hospital,Tianjin University of Traditional Chinese Medicine;Department of Pathology,First Teaching Hospital,Tianjin University of Traditional Chinese Medicine;Graduate School,Tianjin University of Traditional Chinese Medicine;;Effect of green and red powder enema on serum LPS,TNF-α and apoptosis of colon epithelial cells in ulcerative colitis rats[J];中国中西医结合消化杂志;2017-12
5 Ji Manyan;Zeng Kun;Wang Hua;Maternal and Child Health Hospital of Shiyan City;Beijing University of Chinese Medicine;;Simultaneous Determination of Seven Alkaloid Constitutents in Sophora Flavescens Ait. by HPLC-DAD[J];中国药师;2017-01
6 ZHOU Zheng-hua;YUE Yan;WANG Wei;WANG Hong-xia;KANG Hong-chang;WEI Jing-jing;HU Xi-bao;JI Jian-bin;The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine;;Clinical observation on the treatment of ulcerative colitis by retention enema with Green and red powder combined with Mesalazin Enteric-coated tablets[J];天津中医药;2016-12
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