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《CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY》 1998-01
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Detection of rotavirus RNA using DIG labelled probe prepared by polymerase chain reaction

Fu Wanhai, Hao Wei, Peng Yijun. Department of Pediatrics of Zhujiang Hospital. First Military University, Guangzhou 510282  
The digoxigenin (DIG) labelled cDNA probe of rotaviruse was directly prepared by polymerase chain reaction(PCR). The results of cDNA-RNA hybridizaton showed that the DIG-cDNA probe exhibits rotavirus specificity and can detect as tiny as 10pg rotavirus RNA. 120 fecal samples of diarrhea from infants and young children were tested by dot-blot hybridization. It was shown that the positive rate of dot-blot hybridization(65.0%)was significantly higher than that of PAGE(49.1%). This study indicated that direct preparation of DIG-labelled rotavirus-cDNA probe by PCR is much faster and simpler than common method of labeling. 
【CateGory Index】: R373,
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【References】
Chinese Journal Full-text Database 2 Hits
1 LI Ning, YAO Yingmin. Department of Pediatrics,Nanfang Hospital, the First Military Medical University, Guangzhou 510515, China;Multi-system rotavirus infection in 2 infants[J];中华儿科杂志;2002-03
2 LI Ning, YAO Ying-min, Wen Shu-juan (Department of Paediatrics, Department of Endocrinology, Nanfang Hospital, First Militia Medical University, Guangzhou 510515, China);Viremia in infants with rotavirus diarrhea: a preliminary study[J];第一军医大学学报;2001-04
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Fu Wan-hai,Peng Xuan-jun,zhuo Li-mei,et al(First Military Medical U niversity Zhujiang H ospital,Guangzhou 510282);DETECTION OF ROTAVIRUS FROM ASYMPTOMATIC NEONATAL INFECTION WITH POLYMERASE CHAIN REACTION[J];中华微生物学和免疫学杂志;1994-01
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 Fu Wanhai, Hao Wei, Peng Yijun. Department of Pediatrics of Zhujiang Hospital. First Military University, Guangzhou 510282;Detection of rotavirus RNA using DIG labelled probe prepared by polymerase chain reaction[J];中华实验和临床病毒学杂志;1998-01
2 康润田;The Preparation of Nonradioactive EGF-antisense mRNAProbes and in Situ Hybridization with Tissue Section[J];长治医学院学报;1994-03
【Co-references】
Chinese Journal Full-text Database 5 Hits
1 Li Xinming, Li luping, Liu Jun, et al (Department of Microbiology, School of Basic Medical Sciences, China Medical University, Shenyang, 110001);Detection of HBV DNA in Mononuclear Cells from Peripheral Blood among Patients with Hepatitis B Virus Infection[J];中国医科大学学报;1998-06
2 Fu Wanhai, Hao Wei, Peng Yijun. Department of Pediatrics of Zhujiang Hospital. First Military University, Guangzhou 510282;Detection of rotavirus RNA using DIG labelled probe prepared by polymerase chain reaction[J];中华实验和临床病毒学杂志;1998-01
3 Song Haijing, Gu Zhiyuan, Lu Yanping. Molecular Biology Laboratory, PLA General Hospital Beijing 100853;Detection of human cytomegalovirus DNA in brain tissues embedded in paraffin by in situ polymerase chain reaction[J];中华微生物学和免疫学杂志;1997-06
4 Yao Yingmin, Zhao Feng, Wu Jianchun, et al. Nanfang Hospital, Guangzhou 510515;A case report of systemic infection caused by rotavirus[J];中华儿科杂志;1997-07
5 Zhou Yuanping 1 Peng Wenwei 1 Yao Jilu 1 Zhang Zhigang 2 Luo Guanghan 3 Deng Zhide 1 Gu Xiuhua 1 (1 Department of Infectious Diseases, 2 Pathology, Third Affiliated Hospital, Sun Yat sen University of Medical Sciences, Guangzhou, 510630,;DETECTION ON HCV RNA IN PARAFFIN EMBEDDED LIVER TISSUES BY IN SITU POLYMERASE CHAIN REACTION[J];中山医科大学学报;1997-02
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 Shi Xuewen;Chen Xiangqian;Yin Mingdong;Cao Qing;Department of Infectious Disease,Sihong Children Hospital;;Dynamic variation of creatine kinase MB isoenzymes and diagnostic value of myocardial damage in children with rotavirus diarrhea[J];国际儿科学杂志;2015-04
2 SUN Rui-rui;LIU Xiao-dong;LUAN Meng-fu;Department of Pediatrics,Weifang Medical University;;The Influencing Factors for Rotavirus Enteritis Complicated with Myocardial Damage in Children[J];中国全科医学;2015-04
3 LI Jian-an;YAN Neng-rong;WU Jian-wu;Shiyan People’s Hospital of Baoan District in Shenzhen City;;The Clinical Analysis on Renal Damage of Infantile Rotavirus Enteritis[J];中国医学创新;2014-29
4 Huang Xiaoya;Ruan Danqing;Zhang Qinbi;Zheng Yuandi;Yang Xiaochun;Department of Pediatrics,the Third Hospital of Yueqing City;;Clinical analysis of rotavirus enteritis in children in Yueqing city[J];中国小儿急救医学;2014-07
5 ZHANG Wei-guo,CHEN De-ping,ZHOU Ai-hua,YAN Jun,WANG Qing (Affiliated Xiangdong Hospital of Hunan Normal University,Liling 412200,P.R.China);Significance of PCT,IL-6 and TNF-α in children with rotavirus enteritis complicated organ impairment out of the intestinal tract[J];山东医药;2013-02
6 LEI Yong.The people’s hospital of daxian pediatrics,dazhou, sichuan 635000, China;Clinical analysis of the Myocardial Injury Caused by Rotavirus Enteritis in Infants[J];中国现代药物应用;2012-15
7 GONG Xiangying (Yiwu Central Hospital, Zhejiang 322000 China);Changes of myocardial enzymes, troponin, and alanine aminotransferase of children with rotavirus enteritis and its clinical analysis[J];浙江医学教育;2012-02
8 ZHANG Wei-guo1,TANG Fang1,CHENG De-ping2,ZHOU Ai-hua1,YAN Jun1,WANG Qing1(1.Department of Pediatrics,the Affiliated Xiangdong Hospital of Hunan Normal University,Liling 412200,Hunan Province,China;2.Department of Clinical Laboratory,the Affiliated Xiangdong Hospital of Hunan Normal University,Liling 412200,Hunan Province,China);Changes of Procalcitonin,Interleukin-6,Tumor Necrosis Factor Alpha Levels in Children with Sepsis for Rotavirus Infection and Their Significances[J];实用儿科临床杂志;2012-06
9 WANG Xiao-Mei,YU Xiao-Hong,AI Hong-Wu.Department of Clinical Laboratory,Wuhan Medical and Health Center for Women and Children,Wuhan 430016,Hubei,China;Change of serum Cystatin C level in children with rotavirus A enteritis[J];中国妇幼保健;2011-33
10 CAI Xue-mei.Department of Gastroenterology,Wulumuqi Children's Hospital,Wulumuqi 830002,Xinjiang,China;A Clinical Analysis of 192 Cases of Rotavirus Gastroenteritis[J];中华全科医学;2011-10
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