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《Chinese Journal of Neurosurgery》 2003-04
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Establishment of an animal model of carotid atherosclerotic stenosis

XU Yong-ge,ZHOU Ding-biao,ZHENG Ji-yi,et al.Department of Neurosu rgery,General Hospital of PLA,Beijing100853,Chi na  
Objecti ve To establish the methods and factors affecting the development of an economica l,practical,stable and reliable animal model suitable for experimental studies of surgical treatments of carotid atherosclerotic stenosis(CASS).Methods Us ing25New Zealand rabbits,lesions were created by air-drying isolated segment s of carotid arteries(120ml ?min×15min)and feeding the rabbits a diet o f2%cholesterol,6%peanut oil for15days,1month,2months and3months sep arately.Digital subtraction angiography was used to evaluate the stenosis of ca rotid arteries.Many histo logical methods,such as hematoxylin staining瑂pecia l staining and several kinds of immunohistochemical analyses,were used to illus trate the characteristics of the atherosclerotic lesions,and then to screen out the best method to develop the CASS animal model.Results The average rates of stenosis were calcu lated and described as following,half-month group:46. 32%±19.22%;1-month group:72.80%±19.28%;2-month gro up:73.47%±22.26%;and3-month group:59.19%±25.22%. The percentages of moderate to severe stenosis of the4groups were all more than 80%,and the percentage of severe stenosis of the last3groups were closed to or more than50%.The pathological studies suggested that the atheroscle roti c lesions getting more and more severe as the feeding time went on.Fed the at herogenic diet for15days,the lesions fell into the fatty streak period of ath erosclerosis;fed for1month,the lesions belong to the early fibrous plaque per iod;fed for2month,the lesions fell into the mature fibrous plaque period;and fed for3month,the lesions were belong to the early atheromatous plaque period .Conclusion Injured and fed as described by the authors,the animal model of2-month group is stable and reliable,and it meets the demand of experimental st udies of surgical treatments of CASS.
【Fund】: “九五”国家重点科技攻关项目(96-906-02-25);; “九五”军队医药卫生科研基金(96Z058)
【CateGory Index】: R-332
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