Apoptosis of hepatocytes in rat of obstructive jaundice and expression of apoptotic related genes bcl-2 and bax
WANG Jianming *, SUN Hongwu, ZOU Qian, et al. *Department of Surgery, Tongji Hospital, Tongji Medical University, Wuhan 430030, China
Objective\ To explore the regulating mechanism of hepatic injury in obstructive jaundice (OJ). Methods\ The apoptotic status and the expression of apoptotic related gene bcl 2 and bax protein in liver of OJ were detected by using terminal deoxynucleotidyl mediated nick end labeling (TUNEL) and immunohistochemistry method. Results\ The apoptotic rate of liver was related to the duration of OJ. Apoptosis reached the highest after 14 day bile duct ligation, apoptotic index (AI) was 58.23±1.58 ( P 0.05). The stronger bcl 2 expression was, the weaker bax expression and the less number of apoptotic cells in OJ. Conclusion\ Both bcl 2 and bax might take part in the regulation and the development and progression of hepatic injury in OJ.