The dynamic effect of sevoflurane anesthesia on ATPase activity in rat brain
CHEN Chuan-yi ~*, PENG Ren-xiu, LI Yuan-tao. ~* Department of Pharmacology, Medical College, Wuhan University, Wuhan 430071, China
Objective To observe the changes of Na~+, K~+ -ATPase and Ca~(2+)-ATPase activity in different brain regions of rat at different anesthesia stages duringsevoflurane anesthesia and explore the role of ATPase in the anesthetic mechanism of sevoflurane. Methods Forty rats weighing 250-300 g were randomly divided into five groups of 8 animals each, control, induction of anesthesia, maintenance of anesthesia, recovery from anesthesia and complete recovery. Rats were placed in a special glass anesthesia box and exposed to 2.5% sevoflurane. The induction of anesthesia started from staggering of the animal to loss of righting reflex. The maintenance of anesthesia was from 1min after loss of righting reflex. The recovery of anesthesia lasted from recovery of righting reflex to staggering after sevoflurane anesthesia was discontinued and glass box was opened and the complete recovery was defined from 1h after recovery of righting reflex. The animals were decapitated at different stages of anesthesia. Cerebral cortex, hippocapus and brain stem were immediately removed on ice and frozen in liquid nitrogen. The Na~+, K~+-ATPase and Ca~(2+)-ATPase activity in different brain regions of rat at different anesthesia stages were measured by spectrophotometer. Results The Na~+ , K~+-ATPase activity in cerebral cortex, brain stem and hippocampus begun to decrease during the induction of anesthesia and reached the lowest level during maintenance of anesthesia (P0.01 ), begun to increase during recovery from anesthesia and returned to preanesthetic level during complete recovery (P0.05). The Ca~(2+)-ATPase activity in different brain regions begun to decrease during the induction of anesthesia (P 0.05 or P 0.01 ) and reached the lowest level during maintenance of anesthesia (P 0.01 ), begun to increase during recovery from anesthesiaand returned to the normal level during complete recovery (P 0.05 ). Conclusion Sevoflurane significantly inhibits the Na~+, K~+ -ATPase and Ca~(2+) -ATPase activity inthe brain, which is closely related to the depth of anesthesia, which suggest that Na~+, K~+ -ATPase and Ca~(2+) -ATPase in central nervous system may be pharmacologictargets for sevoflurane and be related to the mechanism of sevoflurane anesthesia.
【CateGory Index】： R614