Expression of Peroxisome Proliferator-actived Receptor Y in Renal Cell Carcinoma
YANG Feng-guang, SHI Chang-jin, ZHANG Zhi-wen, et al. Department of Urology, Frist Hospital, Peking University, Institute of Urology, Peking University, Beijing 100034, China
Objective To investigate the expression of peroxisome proliferator-actived receptor γ (PPAR-7) in renal cell carcinoma and to evaluate its possible significance. Methods The expression of PPAR-y mRNA and protein was detected in NK (normal kidney) tissues, RCC (renal cell carcinoma) tissues, two RCC-derived cell lines (786-O and A498) and two NK-derived cell lines HK-2 and HMCC by using RT-PCR and Western blot analysis. Immunohistochemistry was performed on specimens from 120 patients with RCC and from 20 NK tissues to examine the expression and localization of PPAR-γ. Results PPAR-γmRNA was expressed in all NK, RCC tissues, two RCC cell lines and two NK cell lines. The level of PPAR-γ protein was about 7.0-10.7 folds in RCC compared to NK by Western blot analysis. Immuno-histochemical study showed that PPAR-7was markedly increased in renal cell carcinoma tissues (t = 7.888, P 0.01)with overall positive rate of 98.3 % . PPAR-γ was strongly expressed in the perinuclear and cytoplasmic regions in RCC tissues. PPAR-γ expression was correlated with the stage and grade of RCC. Conclusion The expression of PPAR γ is stronger in RCC than in NK. PPAR-γ may play a role in the pathogenesis and progression of RCC. Actived PPAR-γ may he a novel approach to the treatment of this refractory neoplasm.