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An experimental study of the effect of TGF β_1 on the collagen expression by renal interstitial fibroblasts

WANG Weiming, YAO Jian, SHI Rong, et al. Department of Nephrology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025  
Objective To investigate the effect of TGF β 1 on the proliferation, collagen synthesis and collagen mRNA type Ⅰand type Ⅲ expression of renal interstitial fibroblasts in vitro. Methods Fibroblasts were isolated from rat renal medulla. Collagen synthesis and expression of collagen type Ⅰand type Ⅲ mRNA of renal interstitial fibroblasts were investigated by L 3 H proline incorporation and RT PCR, respectively. Results TGF β 1 had a dose dependent stimulating effect on the collagen synthesis of fibroblasts with or without the presence of serum (r= 0 9822, P0 05 and r = 0 9597, P0 05, respectively). TGF β 1 could stimulate the proliferation and collagen synthesis of renal interstitial fibroblasts. By the RT PCR method,TGF β 1 could increase the expression of collagen type Ⅰ and type Ⅲ mRNA of fibroblasts with dose dependent (from 2 to 10 ng/ml) and time dependent effects (from 6 to 48 hours with the concentration of TGF β 1 of 5 ng/ml).Conclusion TGF β 1 at a low concentration may be involved in renal interstitial fibrosis by promoting the collagen synthesis and expression of fibroblasts.
【CateGory Index】: R363
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