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《Chinese Journal of Surgery》 2004-06
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Effects of substance P on granulation tissue fibroblasts proliferation and expression of basic fibroblast growth factor mRNA

JIANG Wei, WANG Zheng guo, LAI Xi nan, ZHU Jin ming,ZHU Pei fang. Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China  
Objective To explore the proliferation promoting effect of sensory neuropeptide substance P (SP) on the cultured granulation tissue fibroblasts in vitro and its regulative effect on the gene expression of basic fibroblast growth factor (bFGF) mRNA. Methods The proliferation promoting effect of cultured granulation tissue fibroblasts was observed by means of MTT; the regulative effect of SP on gene expression of fibroblast bFGF by RT PCR. The time and dose efficiency relations were also observed. Results There was a significant proliferation promoting effect of SP on the cultured granulation tissue fibroblasts in vitro in a remarkable dose dependent fashion. However, bFGF antibody only partly exerted its inhibitive effect. SP could induce the bFGF mRNA expression of the fibroblasts at the 3rd and 6th hour ( P 0 01). SP could promote the bFGF mRNA expression of the fibroblasts in the concentration of 10 -9 -10 -5 mol/L and peaked in the concentration of 10 -7 mol/L. Conclusions SP has a significant proliferation promoting effect on the granulation tissue fibroblasts, which is correlated with SP inducing bFGF mRNA expression of fibroblasts.
【Fund】: 国家重点基础研究发展规划资助项目 (G1990 5 42 0 4)
【CateGory Index】: R605
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