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《Chinese Journal of Microbiology and Immunology》 2003-09
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Polymorphism of human cytomegalovirus UL136 gene in low passage clinical isolates

RUAN Qiang, LU Ying, HE Rong, JI Yao-hua, QI Ying, LIU Qing, CHEN Shu-rong, MA Yan-ping. Virus Laboratory, The 2nd Clinical Hospital, China Medical University, Shenyang 110004, China  
Objective To investigate the polymorphism of human cytomegalovirus UL136 gene in low passage clinical isolates and to find the relationship between the polymorphism and different pathogenesis of congenital HCMV infection. Methods PCR amplification products of 12 isolates were directly sequenced and analyzed. Results 12 of 48 isolates were successfully amplified with positive rate of 25%. By comparison with Toledo sequence, the length of UL136 ORF in all 12 clinical isolates was similar to that of Toledo, 723 bp in size. They had the potential to encode 241 amino acid protein. DNA sequence variations were nucleotide substitutions, neither insertions nor deletions were found. Alignment comparison of clinical isolates UL136 sequences with that of Toledo revealed nt and aa sequence homologies of 97.7%-99.3% and 96.6%-99.1%, respectively. Amino acid variability rate of UL136 protein was 0.83%-3.3%. Two types of secondary structure have been found. Most posttranslational modification sites of UL136 protein were highly conserved although several strains had deleted or additional sites. The phylogenetic trees based on UL136 genes of Toledo strain and 12 clinical isolates demonstrated that 45J was the most closely strain related to Toledo. Conclusion All DNA and deduced amino acid sequences of UL136 gene shared similarity among HCMV clinical strains regardless of their polymorphism. No linkage was found between diversities of UL136 gene and the outcomes of congenital HCMV infection.
【Fund】: 国家自然科学基金资助项目 (3 0 170 986)
【CateGory Index】: R346
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