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《Chinese Journal of Microbiology and Immunology》 2004-03
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The polymorphism of human cytomegalovirus UL138-UL142 genes in low passage clinical isolates

QI Ying, LU Ying, MA Yan ping, RUAN Qiang, HE Rong, JI Yao hua, LIU Qing, CHEN Shu rong. Virus Laboratory, 2nd Clinical Hospital, China Medical University, Shenyang 110004, China  
Objective To investigate the polymorphism of human cytomegalovirus UL138 UL142 genes in low passage clinical isolates and to find the relationship between the polymorphism and pathogenesis of congenital HCMV infection. Methods PCR was performed to amplify the entire UL138 UL142 genes region of clinical isolates, which had been proven containing detectable HCMV DNA. The PCR products in clinical isolates were sequenced directly and the sequence data were analyzed. Results HCMV UL138, UL142 ORFs in clinical isolates were highly conserved; UL139 ORF was hypervariable and was clustered into 3 major genotypes, these changes were concentrated in the 5′ half of the UL139 ORF; by comparison with that of Toledo, UL140 ORF in all clinical isolates had an insertion and their length was 231bp longer than that of Toledo; UL141 ORF in all clinical isolates had an deletion, so there were two new ORFs(UL141a and UL141b). Sulfation cAMP phosphorylation site(SPS) and Casein kinaseⅡ phosphorylation site(CKP) were two new potential functional sites of UL140 protein in clinical isolates. Conclusion The 5′ half of the UL139 ORF in clinical isolates was hypervariable and the nucleotide sequences could be classified into 3 major genotypes, so it might play an important role in the pathogenesis of congenital HCMV infection; Up to the present, no definite relation had been ascertained between some gene in UL138 UL142 region and the virulence of studied HCMV strains.
【Fund】: 国家自然科学基金资助项目 ( 3 0 170 986)
【CateGory Index】: R373
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