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Genotyping of Pseudomonas aeruginosa by randomly amplified polymorphic DNA fingerprinting

Guo Yongjian *, Zhu Zhongyong. *Department of Clinical Microbiology,Fujian Medical University,Fuzhou 350004  
Randomly amplified polymorphic DNA fingerprinting was established and used to type 75 epidemiological related or unrelated strains of Pseudomonas aeruginosa .Typeability was 100%.Thirty two epidemic strains from the newborns were divided into 4 serotypes and 5 RAPD profiles;among which 25 strains had a same profile.The first and the third isolate from patient C were the same both in serotype and RAPD profile,but different from the second one both in serotype and RAPD profile.The follow up strains from each of 7 other patients had a same profile.Thirteen sporadic isolates were divided into 3 serotypes and 10 RAPD profiles.Twelve epidemiological unrelated strains were distinguishable each other in RAPD profiles.The results showed that RAPD fingerprint genotyping is suitable for studies on the etiology,pathogenesis and epidemiology of microbe infection at molecular level with high typeability,powerful discrimination,rapidness and simplicity.
【Fund】: 福建省卫生厅科学基金
【CateGory Index】: R378.991
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