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《Chinese Journal of Hematology》 2002-11
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Role of Th1 cell in the pathogenesis of aplastic anemia

HE Hong, SHAO Zonghong, HE Guangsheng, LIU Hong, SHI Jun, FU Rong, ZHAO Mingfeng, BAI Jie, JIA Hairong, SUN Juan, CUI Zhenzhu, CHU Yulin, YANG Tianying, YANG Chongli. Institute of Hematology and Blood Diseases Hospital,CAMS and PUMC, Tianjin 300020, China  
Objective To detect the quantity, proportion and function of producing cytokines of Th1 and Th2 cells in aplastic anemia (AA) patients and their contribution to the hematopoietic failure. Methods ①Eleven patients with severe aplastic anemia(SAA) at diagnosis were observed by Marsh's method for the CFU E, BFU E and CFU GM before and after depletion of CD 4 + T lymphocytes from bone marrow mononuclear cells (BMMNC); ②Th1(CD 4 +IFN γ +) and Th2 (CD 4 +IL 4 +) cells in peripheral blood mononuclear cells (PBMNC) of 21 SAA patients and 17 normal controls were counted by FACS. ③mRNA expression of IFN γ and IL 4 gene in unstimulated BMMNC from 16 SAA patients, 11 chronic aplastic anemia(CAA) patients,26 other hematological diseases patients and 11 normal controls were measured by reverse transcriptase polymerase chain reaction (RT PCR). Result ①CFU E,CFU GM and BFU E increased significantly after depletion of CD 4 + T lymphocytes from BMMNC of SAA patients. ②The percentage of IFN γ producing CD 4 + T cell (Th1) of SAA patients was significantly higher than that of controls, the percentages of IL 4 producing CD 4 + T cells (Th2) had no difference between SAA patients and normal controls. ③IFN γ mRNA was detected in unstimulated BMMNC in 13 of 16 SAA patients, 6 of 11 CAA patients and one of 6 paroxysmal nocturnal hemoglobinuria (PNH) patients. The IFN γ mRNA was not detected in unstimulated BMMNC of 11 normal controls and other hematological diseases patients. Conclusions Disbalance of CD 4 + T lymphocytes subsets and increases in quantity and IFN γ producing function of Th1 cells might be important for the development of bone marrow failure in AA and in distinguishing AA from other kinds of pancytopenic diseases.
【Fund】: 天津市自然科学基金资助项目 (0 0 3 60 611)
【CateGory Index】: R556.5
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