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《Chinese Journal of Hematology》 2004-04
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Study on the T lymphocytes early activation and soluble tumor necrosis factor receptor in patients with aplastic anemia

SHEN Rong,XU Cong-gao,LI Li-zhen,ZHANG Ti,QIN Xue-mei,LI Jie,ZHAO Chuan-li. Qilu Hospital of Shandong University,Jinan250012,China Corresponding author:XU Cong-gao  
ObjectiveTo investigate the expression of T cell early activation marker (CD 69) on peripheral CD 4 +and CD 8 +lymphocytes and serum levels of soluble tumor necrosis factor receptor 1 and 2 ( sTNF-R1 and sTNF-R2) in serum and bone marrow in patients with aplastic anemia (AA) and their pathophysiological significance. Methods In vitro activation of T lymphocytes was carried out by whole blood cell culture containing PHA (20 μg/ml). The CD 69 expressions on CD 4 + and CD 8 +lymphocytes at 0 h and 4 h after PHA exposure were analyzed by two-color flow cytometry. The levels of sTNF-R1 and sTNF-R2 in serum and bone marrow were measured by ELISA. Results The CD 69 expression rates of CD 4 +and of CD 8 + cells in SAA patients were (8.96±7.23)% and (10.67±7.58)%,respectively, and that of CD 8 +cells in CAA patients was (7.36±5.49)% before PHA stimulation. The CD 69 expression rates of CD 4 +and of CD 8 +cells in SAA patients were (71.73±11.91)% and (61.74±13.44)% and in CAA (59.35±10.15)% and (48.78±8.25)% respectively, and were significantly elevated after PHA stimulation.CD 69 expression on CD 4 +cells was much higher than that on CD 8 +cells after stimulation.The levels of the two sTNF-R (sTNF-R1 and sTNF-R2) in peripheral blood and bone marrow of SAA patients were elevated and in the bone marrow of CAA patients were also increased.The serum levels of sTNF-R2 were positively related to the CD 69 expression rates of CD 8 +cells before PHA stimulation. Conclusion Increased early activation and activated potentials of T lymphocytes,along with abnormally elevated immunologically active molecules might play a major role in the pathogenesis of AA.
【Fund】: 山东省自然科学基金资助项目 (Y2 0 0 2C0 7)
【CateGory Index】: R556.5
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