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Reference limits of the reticulocyte counts

Peng Mingting~* Li Xueguang Wang Wei. *National Center for Clinical Laboratories,Beijing 100730  
Objective To establish a reference limit of the reticulocyte counts and its classification. Methods The reticulocytes were counted by a flow cytometer (Sysmex R-3000) with uramine-0 as fluorochrome.The data presented describe the counting of reticulocytes in 701 apparently healthy sub- jects of both sexes who came for a periodic health examination.According to age and sex,all subjects were divided into four groups.Results In men aged 20~60 yr old,the percentage of reticulocyte in total erythrocytes(Ret)and the reticulocyte count per liter(Ret~#)were 0.0117±0.0036(x~-±s)and (0.0545±0.0177)×10~(12)/L(x~-±s)respectively.In 20~60 yr old female,Ret % and Ret~# were 0.01 14±0.0037 and(0.0473±0.0166)×10~(12)/L respectively.The Student's t-test showed that there were statistically significant differences between men and women aged 20~60 yr or between aged 20~ 60 gr and aged over 60 in women.Conclusions Reference limits of the reticulocyte counts(percent- age and absolute value)and classification(three types of reticulocytes)according to age and sex are pro- vided.
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