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《Chinese Journal of Laboratory Medicine》 1999-04
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Plasmid profiles, drug resistant activities and genetically homologous correlation of 130 strains of Shigella.

BAO Qiyu, ZHOU Liping, WU Shuzhen, et al. Department of Microbiology and Immunology, Wenzhou Medical College, Wenzhou 325003  
Objective To analyze the plasmid profiles, drug resistant activities and genetically homologous correlationship of 130 strains of Shigella.Methods Agarose gel electrophoresis analysis was used to detect plasmid profiles. Plate transconjugation and broth transconjugation were used to examine the conjugative resistant plasmids and the agar plate dilution method was used to analyze the minimum inhibitory concentration (MIC) of 15 antimicrobial agents. Results All (130) strains contained 5 small molecular weight plasmids. (One was larger than 6×10 6 and the other four were smaller thant 6×10 6) 128 strains contained a large plamised (about 120×10 6) and 18 strains harbored one or two middle sized plasmids (34~80×10 6). The results of MIC of 15 antimicrobial agents showed that all the strains were sensitive to ceftriaxone, amikacin, cefoperazone cefazolin and gentamycin. The susceptibility rates were in the range of 100%~88.6%. The range of MIC 90 was2~ 10 μg/ml. The susceptibility rates of the strains to other 10 agents were nearly or equal to 0%. Different plasmid profile strains had almost the same antimicrobial susceptibilities. 25 strains (including 18 strains harboring 34~80×10 6 plasmids) selectively examined to have no self transmissible conjugative plasmid.Conclusion Drug resistant activities of the Shigella were carried by the small plasmids and most of the strains had geneticlally homologous correlation.
【Fund】: 浙江省教委基金
【CateGory Index】: R378.25
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