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Mearurement of free amino acid of biological fluids by rcverscd一phasc HPLC and clinical applica-tion

Tang Zhi-yi;Xu Wei-gui;Yang Zhen-hua;et al.(Beijing Hospital,Beijing 100730)  
he method of simultaneous determination of 29 free amino acid(FAA)in human serum,urine and cerebrospinal fluid(CSF)by reversed一Phase liquid chromatograghy was reported.The sample mixed with Methionine Sulfone as internal standard was derivated with PITC and detected with a UV一detector at 254nm。The linear range of the detection was 10~1000umol/L,the correlation coefficients between the ratio of peak area of FAA to internal standard and FAA concentration were above 0.994.The limit of detection was 2umol/L. The coefficient of variation within group was 1.5%~48%。The coemcient of variation among groups was 3.2%~5.1%。 The recovery rate was 91.12%~103.21 %。 The concentration of FAA in sera of 15 healthy adults and 18 nephropathic pa-tients were determined;the results showed that both had very marked difference in the ratio of necessary amino acid and unnecessary amino acid(P0.001)。
【CateGory Index】: R692.04
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1 YANG Sheng-yuan;JIANG Min;HU Xiao-jun;LIU Shu-min;WANG Sheng;College of Chemistry and Chemical Engineering,Lingnan Normal University;Tropical South China Sea's Resources Collaborative Innovation Centre,Lingnan Normal University;;A Novel Edible Material with Rich Natural Gamma-aminobutyric Acid[J];食品研究与开发;2017-22
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