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《Chinese Journal of Ophthalmology》 2001-01
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Apoptosis of retinal capillary cells in diabetic rats and expression of apoptosis-related genes

LIU Xuezheng , XIAO Hong, QU Weixin, et al  Department of Anatomy, Jinzhou Medical College, Jinzhou 121001, China  
Objective To determine the apoptosis of retinal capillary cells in early diabetic rats and observe the expression of apoptosis related genes (bcl 2, Bax) in retinas of early diabetic rats Methods 40 healthy adult Wistar rats were chosen and randomly divided into four groups: normal control (CON), diabetes mellitus one month (DM1), three months (DM3) and six months (DM6) Diabetic mellitus in rats was induced by streptozotocin (STZ) intraperitoneally Retinal preparations of blood vessels and paraffin sections were stained by TdT mediated dUTP nick end labeling (TUNEL) and immunohistochemical (ABC) method respectively and analyzed by computer picture analytic system on the results of ABC method Results Positive pericyte nuclei labeled by TUNEL were emerged in DM3 and DM6, but positive endothelial cell nuclei only in DM6 No positive reaction was seen in CON and DM1 Chromatin of TUNEL positive nuclei was distributed unequally, showing apoptotic characteristics of ring like and crescent nuclei In ABC staining method, two genes′ (bcl 2, Bax) proteins were expressed in retinal vessels Along with the progression of diabetes, the intensity of positive reaction was increased in degrees Moreover, pigment epithelial cells in DM3 showed Bcl 2 positive reaction that again extended further to inner rod segments and ganglion cells in DM6, while in DM6, Bax was expressed in ganglion cells Conclusions The nature of pericyte dropout in early diabetic rats is apoptosis that also exists in endothelial cells; the expression of Bax and bcl 2 in retinas of early diabetic rats is enhancing
【Key Words】： Diabetic retinopathy Capillaries Cell death Gene expression
【CateGory Index】： R587.2
【DOI】： CNKI:SUN:ZHYK.0.2001-01-030
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