Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《National Medical Journal of China》 2003-08
Add to Favorite Get Latest Update

The study of cis-element HNF4 in the regulation of mfgl2 prothrombinase/fibroleukin gene expression in response to nucleocapsid protein of MHV-3

NING Qin , LUO Xiao-ping,WANG Zhi-mo, HAN Mei-fang, YAN Wei-ming, LIU Ming-feng, Gary Levy. Division of Clinical Immunology, Tongji Hospital, Institute of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaCorresponding author: NING Qin, Email: qning@tjh.tjmu.edu.cn  
Objective To identify the transcription factor(s) that is essential for activation of mfgl2 prothrombinase/fibroleukin gene in response to nucleocapsid protein of murine hepatitis virus type 3 (MHV-3). Methods Western blotting was performed to investigate whether HNF4 is expressed in macrophages of Ba1b/c mice where mfgl2 is expressed. Confocus microscope immunofluoresence was performed to show whether N protein of MHV enters into the nucleus of infected cells, which is a critical step for the N protein to facilitate its transactivation property. To facilitate the identification of three candidate factor(s) including hepatocyte nuclear factor 4 (HNF4)/liver factor A1 (LF-A1), cytomegalovirus immediate early gene 1.2 (IE1.2) regulatory element and granulocyte- macrophage colony stimulating factor (GM-CSF) in response to mfgl2 activation upon the stimulation of MHV-A59 N protein, gel mobility shift assay (GMSA), competition experiments and site directed mutagenesis were performed. Results Western blotting displayed that HNF4 was constitutively expressed in macrophages and did not show significant change under the stimulation of different MHV. Confocus microscope immunofluoresence clearly showed that N protein of MHV entered into the nucleus of infected cells. GMSA and competition experiments demonstrated binding to both HNF4 and IE1.2 fragments could be competed with the cold specific oligonucleotides but not with the same amount of non-specific oligos nucleotides. A super shift band was observed when HNF4 antibody was pre-incubated with the nuclear extracts indicating the interaction between the HNF4 element and mfgl2 promoter. Site directed mutagenesis of cis-elements HNF4 (pfgl2HNF4mut) and HNF4/IE1.2 (pfgl2HNF4/IE1.2mut) mutations abolished over 75% of transcription from wild-type mfgl2 promoter. However the pfgl2IE1.2mut displayed almost wild-type promoter activity (75%~80%). Conclusions The factor HNF4 binds to mfgl2 promoter and serves as an essential transcription factor for mfgl2/fibroleukin expression in respons to MHV-3 N protein.
【Fund】: 国家自然科学基金资助项目 (NSFC30 170 846 );; 国家杰出青年科学基金项目 (NSFC30 2 2 5 0 40 NSFC30 12 5 0 19)
【CateGory Index】: R575.3
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 5 Hits
1 YI Wen-long,FANG Feng,NING Qin,YAN Wei-ming,ZHEN Hong,SHU Sai-nan,ZHOU Yu-feng,LI Ge(Laboratory of Pediatric Clinical Virology of Affiliated Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China);Experimental study on the antivirus effect of Shuanghuanglian and herba houttuyniae on mouse hepatitis virus strain Ⅲ in vitro[J];Journal of Southeast University(Medical Science Edition);2006-04
2 YI Wen-long,FANG Feng*,NING Qin1,YAN Wei-ming1,ZHEN Hong,ZHOU Yu-feng,LI Ge(Laboratory of Pediatric Clinical Virology,1Laboratory of Infection Immunity,the Affiliated Tongji Hospital,Tong ji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China);In vitro antivirus effect of allitridin injection on hepatitis virus strain 3 in Mouse[J];Acta Universitatis Medicinalis Nanjing(Natural Science);2006-06
3 Qin Ning,Wei-Ming Yan,Zhi-Mo Wang,Dong Xi, Xiao-Ping Luo, Department of Infectious Disease, Tongji Hospital, Research Institute of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China Ming-Feng Liu, Gary Levy, Toronto General Hospital, The University of Toronto, Toronto, ON M5G 2C4, Canada;Domain I of nucleocapsid protein of murine hepatitis virus strain 3 upregulates transcription of mfg12 prothrimbinase/fibroleukin gene[J];World Chinese Journal of Digestology;2004-03
4 ZHU Fan, NING Qin, CHEN Yue, TAO Xin-xue, YAN Wei-ming, XI Dong, YAN Fuming, HAO Lian-jie, LUO Xiao-ping. Department of Infectious Disease, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology Wuhan 430030, China;Hfgl 2/fibroleukin expression in liver and peripheral blood mononuclear cells (PBMC) and its correlation with disease severity[J];Chinese Journal of Hepatology;2004-07
5 Wen-Long Yi,Feng Fang,Sai-Nan Shu,Ge Li,Department of Pediatric Clinical Virology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei Province,China Wen-Long Yi,Tianyou Hospital,Wuhan University of Science and Technology,Wuhan 430030,Hubei Province,China Qin Ning,Department of Infection and Immunity,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei Province,China;Preventive and therapeutic effects of Shuanghuanglian and allitride against experimental murine hepatitis virus type 3 infection in vivo[J];World Chinese Journal of Digestology;2011-22
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Zhang Yuan 1,2) Qi Yilin 1) Hong Jiong 1) Zheng Guangyu 1) Ruan Li 3) Zheng Nan 1,3) ( 1)College of Life Sciences, Beijing Normal University, 100875, Beijing, China; 2)Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China; 3)National Institute for Viral Disease Control and Prevention , China CDC, 100052, Beijing, China);MOLECULAR PHYLOGENETIC ANALYSIS OF SARS CORONAVIRUS AND RELATED VIRUSES[J];Journal of Beijing Normal University(Natural Science);2003-03
2 YANG Jin-song,CHEN Chun-ru,LI Ju-lian(West China School of Pharmacy,Sichuan University,Chengdu 610041, China);Synthesis of sodium houttuyfonate[J];West China Journal of Pharmaceutical Sciences;2005-01
3 YI Wen-long,FANG Feng,NING Qin,YAN Wei-ming,ZHEN Hong,SHU Sai-nan,ZHOU Yu-feng,LI Ge(Laboratory of Pediatric Clinical Virology of Affiliated Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China);Experimental study on the antivirus effect of Shuanghuanglian and herba houttuyniae on mouse hepatitis virus strain Ⅲ in vitro[J];Journal of Southeast University(Medical Science Edition);2006-04
4 YI Wen-long,FANG Feng*,NING Qin1,YAN Wei-ming1,ZHEN Hong,ZHOU Yu-feng,LI Ge(Laboratory of Pediatric Clinical Virology,1Laboratory of Infection Immunity,the Affiliated Tongji Hospital,Tong ji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China);In vitro antivirus effect of allitridin injection on hepatitis virus strain 3 in Mouse[J];Acta Universitatis Medicinalis Nanjing(Natural Science);2006-06
5 Yang Zhen, Liu Nengyin, Li Dapeng et al Department of Surgery, Tongji Hospital, Tongji Medical University, Wuhan 430030;Classification of the Modes of Hepatic Cell Death Induced by Carbon Tetrachloride, Schistosomiasis and Hepatitis Virus[J];ACTA UNIVERSITATIS MEDICTNAE TANGJI;1999-04
6 Luo Dan, Fang Feng, Zhen Hong et al Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030;Experimental Study of the Influence of Human Serum on the Cytotoxicity and Anti-HCMV Activity of Allitridin[J];Acta Universitatis Medictnae Tangji;2004-02
7 Lü Peng,Luo Hesheng,Yu Baoping Department of Infectious Disease,Jining Municipal Infectious Hospital,Jining 272000;Dynamic analysis of hepatocellular apoptosis and necrosis in liver fibrosis model of rat[J];Chinese Journal of Gastroenterology and Hepatology;2002-03
8 YANG Tie-hong, JIA Min, MEI Qi-bing~(*)Department of Pharmacology, Fourth Military Medical University, Xi’an 710032, China;Effects of Angelica sinensis polysaccharide on cell-mediated immuneity[J];Journal of Cellular and Molecular Immunology;2005-06
9 SU Mei-qiong~1 YANG Bai-chong~2 CHENG Mi-hong~1 LI Yuan-rui~1(1. College of Forestry , Northwest Sci-Tech University of Agriculture and Forestry,Yangling Shaanxi712100, China;2. College of Food Science and Nutrition Engineering, China Agriculture University, Beijing100083, China);Advance in Researches of the Efficacy and Synthetic Utilization of Garlic[J];Acta Agriculturae Boreali-occidentalis Sinica;2003-04
10 Chen Shuanglu(Chen SL),Lu Guojin(Lu GJ)(Tianjin Children's Hospital, Tianjin 300074);Study on antibacterial activity in vitro of six antimicrobial drugs compatible with shuanghuanglian injection[J];Chinese Journal of Modern Applied Pharmacy;1998-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 HUANG Jiaquan,ZHANG Huabing,HUANG Tiejun,et al.Tongji Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;Observation on Effect of Anti-hepatic Failure Complex on Liver Failure in Balb/cJ Mouse Infected With MHV-3 Virus[J];Journal of Internal Intensive Medicine;2007-04
2 XU Ru-ming,DENG Ke-min,LU Yang (Department of Pharmacy,School of Medicine,Shanghai Jiaotong University,Shanghai 200025,China);Protective effect of active component of Eclipta on hepatic injury induced by ConA in mice[J];Journal of Shanghai Jiaotong University(Medical Science);2010-01
3 HUANG Jiaquan,XIAO Fei,YU Haijing,HUANG Tiejun,HUANG Haiyan,NING Qin Department of Infectious Diseases,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;Differential Gene Expression Profiles in Acute Hepatic Failure Model in Mice Infected with MHV-3 Virus Intervened by Anti-hepatic Failure Compound[J];华中科技大学学报(医学英德文版);2007-05
4 LIU Li LIU Min-juan (Department of Hematology the Second Affliated Hospital of Guangzhou Medical College,Guangzhou 510260,China);fg 12 Prothrombinase/Fibroleukin and Hepatitis[J];Chinese Journal of Thrombosis and Hemostasis;2006-05
5 XIAO Fei HUANG Jia-quan YU Hai-jing XI Dong YAN Wei-ming NING Qin Department of Infectious Diseases,Tongji Hospital,Tongji Medical College,Huazhong University of Science & Technology,Wuhan 430030,China;Study on autoimmune hepatitis associated genes by human genome oligonucleotide microarray[J];Chinese Journal of Hepatology;2006-12
6 SU Kai, CHEN Fang, LUO Xiao-ping, NING Qin (Laboratory of Infectious Disease, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, P.R.China);Induced hfgl2 expression in both HUVEC and THP-1 cell lines by IFN-γ and IL-2[J];China Journal of Modern Medicine;2006-16
7 ZHU Yong-fen~1,TIAN De-ying~1,XI Dong~2,et al.1.Division of Infectious Immunology and Department of Infectious Disease,Tongji Hospital of Tongji Medical College,Huazhong University of Science and Technology(Hubei Wuhan,430030)China;Procoagulant activity and expression of a novel procoagulant factor,hfgl2 in patients with severe hepatitis B and its correlation with disease progression[J];Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases;2006-03
8 HUANG Jia-quan,HUANG Tie-jun,ZHANG Jian-jun,et al.Department of Infectious Disease,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology(Wuhan Hubei,430030)China;Clinical effect on severe hepatitis treated by compound anti-fulminant hepatitis[J];Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases;2006-06
9 YUAN HONG1,XIA ZHANG2,CAO TING-ZHI,et al.1.The Third People's Hospital of Shenzhen(Shenzhen Guangdong,518020)China;Research on the model of syndrome differentiation based on different stages for patients with chronic severe hepatitis B[J];Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases;2010-05
10 HUANG Jia-quan1,HUANG Tie-jun1,ZHANG Jian-jun2,HUANG Hai-yan1,YU Hai-jing1,XIAO Fei1,NING Qin1.1.Department of Infectious Diseases,Affiliated Tongji Hospital of Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei,China;2.Department of Infectious Diseases,Zhongshan Hospital,Wuhan 430033,Hubei,China;Comparative study of therapeutic effects of three complex prescriptions of traditional Chinese medicine on mice with acute hepatic failure and on their fibrinogen like protein 2 expression[J];Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care;2007-01
Similar Journals
> Hebei Medical Journal
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved