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《National Medical Journal of China》 2005-04
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Apoptosis of pulmonary alveolar macrophages in aged and adult rats: a comparative study

QIAN Xiao-shun, ZHU Qing-lei, YANG Jie, YIN Tong, WANG Shi-wen. Institute of Geriatric Cardiology, General Hospital of Chinese People′s Liberation Army, Beijing 100853, China  
Objective To investigate the role of alveolar macrophages (AM) in the initiation of multiple organ failure in the elderly (MOFE).Methods Three-month-old (adult) and 24-month-old (aged) males SD rats were used as experimental animals. Zymosan 0.5g/kg was used to establish animal model of MOFE, normal saline were used among the control rats. The rats were divided into 4 groups: aged model group, aged control group, adult model group, and adult control group. Twenty-four hours after the establishment of model, 6 surviving rats from each group were killed. The trachea, bronthi, and lungs were isolated and lavaged with normal saline. One minute later the bronchi-alveolar lavage fluid (BALF) was re-extracted. Cells were collected from the fluid and put into 24-well cell culture plate. The alveolar macrophages (AMs) adhered to the wall were collected, suspended again, cultured, re-collected, centrifuged, and isolated. Apoptosis of the enriched AM was measured by propidium staining and flow cytometry. Fluo-3.AM staining and flow cytometry were used to detect the intracellular free calcium. Mitochondrial membrane electric potential was detected by rhodamine123 staining with flow cytometry. Results The apoptotic rate (APO) of AM in the aged rat models was 43.4%±8.4%, significantly higher than that of adult model rats (24.2%±3.0%, ~P 0.01). Compared with the controls, the intracellular calcium increased , but mitochondrial △Ψm decreased in the 2 model groups. Conclusion The AM APO% of aged MOFE model increases. It may be one of the causes of difficulty to control the inflammation in the lung with MOFE and easiness to induce MOFE in elderly when their lungs are infected or injured. Changes of intracellular calcium and mitochondrial △Ψm may play pivotal roles in apoptosis of AM.
【Fund】: 国家重点基础研究发展规划(973计划)基金资助项目(G2000057004)
【CateGory Index】: R563
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