Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《National Medical Journal of China》 2005-37
Add to Favorite Get Latest Update

The role of local immune response in gastric mucosa in the protection induced by Helicobacter. pylori vaccine with chitosan as adjuvant

XIE Yong*, GONG Yan-feng, ZHOU Nan-jin, CHEN Jiang, ZHOU Xiao-jiang, Lv Nong-hua, WANG Chong-weng. *The First Affiliated Hospital of Jiangxi Medical College, Nanchang 330006, China  
Objective To study the immunological protection of Helicobacter pylori (H. pylori) vaccine with chitosan as adjuvant and it′s mechanism. Methods One-grade female BALB/c mice were randomly divided into nine groups and immunized by ①PBS alone, ②chitosan solution alone, ③chitosan particles alone, ④H. pylori antigen alone, ⑤H. pylori antigen plus chitosan solution, ⑥H. pylori antigen plus chitosan particles, ⑦H. pylori antigen plus CT, ⑧H. pylori antigen plus chitosan solution and cholera toxin (CT), ⑨H. pylori antigen plus chitosan particles and CT orally respectively once a week for four weeks. At 4 weeks after the last immunization, these mice were challenged by alive H. pylori(1×109/ml) twice at two days intervals. At 4 weeks after the last challenge, these mice were all killed and Gastric mucosa were embedded in paraffin, sectioned and assayed with Giemsa stain. The other gastric mucosa were used to quantitatively culture H. pylori. An ELISA was used to detect anti-H. pylori IgA in saliva and gastric mucosa and a quantitative ELISA was used to detect IL-2, IL-4, IL-10 content in gastric mucosa, and SP immunohistochemical method was used to detect secretory immunoglobulin A (sIgA) in gastric mucosa. Results (1)In the groups with chitosan as adjuvant, 60% mice could achieve immunological protection, which was according to that with CT as adjuvant (58.33%), and was significantly higher than H. pylori antigen alone and other groups without H. pylori antigen(P0.01 or P0.05). While the rates of protection in the groups with chitosan plus CT as adjuvant were 84.62%, 85.71% and the H. pylori colonization score in it was significantly lower than that in the groups with CT as adjuvant and without adjuvants(P0.01 or P0.05). (2)the labeling index for sIgA-positive lumen of glands and special anti-H. pylori IgA levels in gastric mucosa in the groups with chitosan as an adjuvant had no difference with those in the group with CT as an adjuvant(P0.05)and were significantly higher than those in non-adjuvant groups, while those in the groups with chitosan plus CT were significantly higher than those in the group with CT as an adjuvant(P0.01 or P0.05). (3)Before challenge, the content of IL-2 in gastric mucosa were no different among different groups(P0.05). After challenge the content of IL-2 were significantly higher in the groups with adjuvant than those in the control group(P0.01 or P0.05), Moreover, those in the groups with antigen after challenge were significantly higher than those before challenge(P0.05). (4)Before challenge, the content of IL-10 in gastric mucosa were significantly higher in the groups with chitosan as adjuvant than those in the control group and the group without adjuvant(P0.01 or P0.05). After challenge, the content of IL-10 were no different among different groups (P0.05). Moreover, those in the groups with adjuvant after challenge were significantly lower than those before challenge(P0.01).(5)Before challenge, the content of IL-4 in gastric mucosa were significantly higher in the groups with chitosan as adjuvant than those in the control group and the group without adjuvant(P0.05),After challenge, the content of IL-4 were significantly higher in the groups with chitosan particles as an adjuvant than those in the group with CT as an adjuvant(P0.05), and those in the group with chitosan solution as an adjuvant were significantly higher than those in control group, non-adjuvant group and the groups with CT(P0.01 or P0.05),Moreover, those in the groups with adjuvant after challenge were significantly lower than those before challenge(P0.01 or P0.05). Conclusions (1)H. pylori vaccine with chitosan as adjuvant could protect against H. pylori infection, this suggested that chitosan could be a mucosa adjuvant of H. pylori vaccine, and it could effectively elicit special humoral immune response of systemic and local mucosa, which might be one of its protective mechanism. (2)H. pylori vaccine with chitosan as adjuvant may induce both Th1 and Th2 type immune response, and after challenge it could reverse the inhibition of Th2 induced by H. pylori infection and recover the Th1/Th2 imbalance. which might contribute to the immune protection against H. pylori.
【Fund】: 国家自然科学基金资助项目(30460052);; 江西省学科带头人培养项目基金资助(K010501);; 江西省卫生厅重点招标项目基金资助(031070);; 江西省教育厅基金资助项目
【CateGory Index】: R392;
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 3 Hits
1 Wang Yi-ting1, Chen Yong2, Hong Yan11Institute of Bioengi- neering, Zhejiang Academy of Medical Science, Hangzhou 310013, Zhejiang Province, China; 2Zhejiang Medical College, Hangzhou 310053, Zhejiang Province, China;Clinical prospect of chitosan as a drug carrier and gene carrier[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2008-10
2 Yong Xie Xiao-Hua Hou Department of Gastroenterology,Union Hospital of Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China Yong Xie Yan-Feng Gong Jiang Chen Nong-Hua Lv Cheng-Yu Mei Institute of Digestive Diseases,the First Affiliated Hospital of Nanchang University,Nanchang 330006,Jiangxi Province,China Nan-Jin Zhou Department of Biochemistry,Jiangxi Institute of Medical Sciences,Nanchang 330006,Jiangxi Province,China;Immune therapeutic effect of Helicobacter pylori vaccine with chitosan adjuvant[J];World Chinese Journal of Digestology;2007-06
3 HUANG Xiang-gang, XU Jian-qing, REN Li, QIU Chao, ZHANG Ning, LIU Lian-xing, WAN Yan-min, PENG Hong, SHAO Yi-ming. National Center for AIDS/STD Control and Prevention, China Center for Disease Control and Prevention,100050 Beijing,China;Intranasal priming with HIV DNA vaccine and systemic boosting with recombinant vaccinia induce vigorous immune responses:experiment with mice[J];National Medical Journal of China;2006-44
【Citations】
Chinese Journal Full-text Database 2 Hits
1 CHEN Jie, CHEN Min hu, LI Guo qing, et al. Department of Gastroenterology, the First Affiliated Hospital, Sun Yat sen University, Guangzhou 510080, China;Th type immune response induced by Helicobacter pylori vaccine in mice[J];Chinese Journal of Digestion;2003-12
2 SHI Tong *, LIU Wen-zhong, SHI Gui-ying,XIAO Shu-dong. *Shanghai Second Medical University, Renji Hospital, Shanghai Institute of Digestive Disease, Shanghai 200001, China;The role of Th1-type response and γ-interferon in the protection induced by Helicobacter pylori vaccine[J];National Medical Journal of China;2003-15
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 GONG Yan-feng, XIE Yong, ZHOU Nan-jin, CHEN Jiang, ZHOU Xiao-jiang, LU Nong-hua, WANG Chong-wen Institute of Digestive Disease, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China;Cellular immunity induced by H.pylori vaccine with chitosan as adjuvant[J];Chinese Journal of Cellular and Molecular Immunology;2007-07
2 Jie Chen, Min-Hu Chen, Jin-Hu Wang, Sen-Lin Zhu, Department of Gastroenterology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China;Influence factors of postimmunization gastritis after Helicobacter pylori vaccine immunization in mice[J];World Chinese Journal of Digestology;2006-23
3 XIE Yong~*,GONG Yan-feng,ZHOU Nan-jin,CHEN Jiang,ZHOU Xiao-jiang,L Nong-hua,WANG Chong-wen.~*The First Affiliated Hospital of Jiangxi Medical College,Nanchang 330006,China;Immunological protection offered by H.pylori vaccine with chitosan adjuvant[J];Chinese Journal of Microbiology and Immunology;2005-10
4 BAI Yang *, ZHANG Ya li, WANG Ji de, ZHANG Zhao shan, ZHOU Dian yuan. *Institute of Digestive Medicine of the People′s Liberation Army, First Military Medical University, Guangzhou 510515, China;Cloning and immunogenicity of conservative region of adhesin gene of Helicobacter pylori[J];National Medical Journal of China;2003-09
5 XIE Yong, GONG Yan-Feng, ZHOU Nan-Jin, CHEN Jiang, ZHOU Xiao-Jiang, L Nong-Hua, HOU Xiao-Hua. The Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Nanchang 330006, China;The role of Th immune response in the protection induced by Helicobacter pylori(Hp) vaccine with chitosa as adjuvant[J];Chinese Journal of Immunology;2006-11
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Yan Jinlong Xu Qi Cai Zhaosheng Yang Chunsheng(College of Chemical and Biological Engineering of Yancheng Technology Institute,YanCheng 224003);Preparation of Chito-oligosaccharide,Its Characteristic and the Relative Development in Its Application[J];Anhui Chemical Industry;2005-01
2 WAN Yi-Yuan1,2, ZHANG Xing1,2, HE You-Jian1,2, JIANG Wen-Qi1,2 1. State Key Laboratory of Oncology in Southern China, Guangzhou, Guangdong, 510060, P. R. China 2. Department of Medical Oncology, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, P. R. China;Feasibility of Chitosan as Gene Therapy Vehicle[J];Chinese Journal of Cancer;2005-11
3 HENG Liying, ZHU Jiang\|feng, SUN Kun\|shan (Institute of Bioengineering Zhejiang Univ.\ 310027, China);Antimicrobial Activity of Chitosan[J];MATERIALS SCIENCE AND ENGINEERING;2000-02
4 Laboratory of Animal Physiology and Biochemistry,Nanjing Agricultural University,Nanjing 210095,China);EFFECTS OF CHITOSAN ON THE PERFORMANCE,LIPIDMETABOLISM,IMMUNE AND ENDOCRINE FUNCTION IN MEAT-TYPE QUAILS[J];Acta Zoonutrimenta Sinica;2001-04
5 WANG Xiu-wu 1,2 ,DU Yu-guang 1 ,BAI Xue-fang 1 ,LI Shu-guang 1 (1.Dalian Institute of Chemical and Physical of Chinese Academy of Science, Dalian116023,China;2.Liaoning Normal University,Dalian116029,China);THE EFFECT OF OLIGOCHITOSAN ON BROILER GUT FLORA,MICROVILLI DENSITY,IMMUNE FUNCTION AND GROWTH PERFORMANCE[J];Acta Zoonutrimenta Sinica;2003-04
6 MA Xiao\|zhen,YANG Ye,XIE Xin\|dong, FENG Yu\|lan (Institute of Animal Husbandry and Veterinary Medicine. Fujian Academy of Agricultural Sciences, Fuzhou,Fujian\ 350003, China);Effect of chitosan on growth performance and lipometabolism of broiler chickens (male)[J];Fujian Journal of Agricultural Sciences;2001-04
7 Liu Yanru (Bioengineering College,Fujian Teachers University,Fuzhou 350007) Yu Ping (Institute of Polymer Science,Fujian Teachers University,Fuzhou 350007);The Water-soluble Chitosan Effects on Immume Function and Transplanted Tumor in Mice[J];JOURNAL OF FUJIAN TEACHERS UNIVERSITY(NATURAL SCIENCE);1999-04
8 LU Meiluan SONG Yufang FU Tianbao ZHENG Senlin QIU Xiaoyan(1 Third Institute of Oceanography, SOA, Xiamen,361005,China;2 Institute of Applied Ecology, Chinese Academyof Sciences, Shenyang,110016,China;3 Department of Biological Engineering, Fisheries College Jimei,University, Jimei, 361002,China;4 Shenyang Key Laboratory of Environmental Engineering, Shenyang University, Shenyang, 100041,China);THE ANTIBIOTICS FUNCTION OF THE CHITOSAN COMBINED AGENT ON THE FEED OF POULTRY[J];Environmental Chemistry;2007-02
9 WU Ping1,2,HE Xiao-xiao1,WANG Ke-min1,TAN Wei-hong1,ZHENG Ming-bin1,PENG Jiao-feng1 (1.State Key Laboratory of Chemo/Biosensing and Chemometrics,Hunan Univ,Changsha,Hunan 410082,China;2.School of Pharmacy,Hunan University of Traditional Chinese Medicine,Changsha,Hunan 410208,China);Preparation of Chitosan Nanoparticles As Protein Drug Carriers[J];Journal of Hunan University(Natural Sciences);2007-09
10 LIU Yan-ru, YU Ping, ZHENG Yi (Bioengineering College, Fujian Teachers' University, Fuzhou 350007);Study on bacteriostatic effect of water-soluble chitosan[J];Chinese Journal of Marine Drugs;2001-02
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 LIANG Ying1 JIANG Ning2 HE Wenjuan3 REN Chengcai2 WANG Deli1 ZHENG Wenfeng1 WANG Juntao1(1.College of Arts and Science,Heilongjiang Bayi Agricultural University,Daqing 163319,China;2.College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China;3.Xilingol Vocational College,Xilinhaote 026000,China);Effects of Astragalus Polysaccharide on Growth Performance and Immune Function of Broilers[J];Chinese Journal of Animal Nutrition;2010-04
2 WANG Zhen-qiang,ZHAO Ren-bang,WANG Xiang-hong,TIAN Yi-ling and LI Hui-ling(Food Science & Technology College of Hebei Agricultural University,Baoding,Hebei 071001,China);Research Progress in the Application of Chitosan in Fruit Wine[J];Liquor-Making Science & Technology;2008-11
3 Wang Yi-ting,Wo En-kangInstitute of Bioengineering,Zhejiang Academy of Medical Sciences,Hangzhou 310013,Zhejiang Province,China;Preparation and characterization of chitosan-pHEV_(23) nanoparticles[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2008-32
4 LIU Bao-chi,LI Lei,LIU Li,et al.(Shanghai Public Health Clinical Center,Shanghai 201508,China);Peri-operative treatment for patients with HIV infection[J];Chinese Journal of AIDS & STD;2010-05
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 CHEN Jie, CHEN Minhu, ZHU Senlin, CHEN Wei. Department of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou (510080);Immunization against Helicobacter pylori Infection with Helicobacter pylori Outer Membrane Protein Vaccine[J];Chinese Journal of Gastroenterology;2001-02
2 LIU Xiaofeng, HU Jialu, HUANG Jiansheng, et al. Institute of Gastroenterology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China;Oral immunization of mice with attenuated Salmonella typhimurium expressing Helicobacter pylori urease B subunit[J];Chinese Journal of Digestion;2001-09
3 ZHU Senlin, CHEN Minhu, LIAO Wenjun, et al. Department of Gastroenterology, the First Affiliated Hospital, Sun Yat sen University of Medical Sciences, Guangzhou 510080, China;Construction and identification of recombinant attenuated Salmonella typhimurium vaccine strain expressing Helicobacter pylori urease subunit B[J];Chinese Journal of Digestion;2001-09
4 CHEN Jie, CHEN Minhu, ZHU Senlin, et al. Department of Gastroenterology, the First Affiliated Hospital, Sun Yat sen University of Medical Sciences, Guangzhou, Guangdong 510080, China;Study of protective immune response induced by attenuated Salmonella typhimurium expressing H.pylori antigens in mice[J];Chinese Journal of Digestion;2002-02
5 WANG Jide, CHEN Ye, LAI Zhuosheng, et al. PLA Institute for Digestive Disease, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China;The evaluation of cagA and other vaccine candidate proteins of Helicobacter pylori on T cell apoptosis mediated by Fas/Fas ligand interaction[J];National Medical Journal of China;2000-11
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved