Growth regulatory mechanisms of recombinant human imterleukin 6 on human lung carcinoma cell lines

Fu Jian,Wu Bingquan,Zheng Jie,et al.Department of Pathology,Beijing Medical University,Beijing 100083  

Objectives To detect the activity of interleukin 6(rhIL 6)on human lung cancer and the influences of recombinant human IL 6(rhIL 6)on the in vitro growth of high metastatic human lung giant cell carcinoma cell line PG and low metastatic human lung adenocarcinoma cell line PAa.Methods The effects of rhIL 6 on the expressions of IL 6,IL 6R,c myc,c fos and c jun mRNAs were analyzed by Northern blotting hybridization.Results rhIL 6 could stimulate the in vitro growth of both PG and PAa cells,in adose and time dependent manner.Both PG and PAa cells were showen to express IL 6 and IL 6Ridentified by reverse transcription PCR analysis.Northern blotting hybridization revealed that rhIL 6up regulated mRNA levels of IL 6,IL 6R and c myc rather than those of c fos and c jun.Conclusion These data suggest that rhIL 6may promote the proliferation of human lung cancer cell lines PG and PAa,which might,at least partly,be related to the up regulation of expressions of IL 6,IL 6 R,c myc gene transcript.

【Key Words】： Lung neoplasmas Interleukin 6 Gene
【CateGory Index】： R734.2

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