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Influence of lipid on antifungal activity in vitro

CHEN Dong-ke,XU Hong-tao(Beijing Hospital of the Ministry of Health,Beijing 100730,China)  
OBJECTIVE To study the influence of lipid on the antifungal activity through comparative analysis of the test results with and without lipid in vitro.METHODS The olive oil,corn oil,sesame oil,soybean oil,glycerin,liquid paraffin,TWEEN20,TWEEN40,TWEEN60 and TWEEN80 were added to the ATB F2 media and every lipid final concentration was made to 0.5% and 2%.MICs of C.krusei ATCC6258,C.parapsilosis ATCC22019 and ATCC14521 were measured by ATB FUNGUS 3 strips.RESULTS Every lipid had no influence on 5-fluorocytosine(5-FC) in the testing of C.krusei ATCC6258 and C.parapsilosis ATCC22019,and to fluconazole(FCA) except the MIC of C.krusei ATCC6258 were 8μg/ml with 2% liquid paraffin oil,other results with different concentrations of lipid paraffin oil were all in quality control range.The four kinds TWEEN on 2% concentration in the testing of C.krusei ATCC6258 had mild inhibition on voriconazole,but to C.parapsilosis ATCC22019 the four kinds TWEEN had no inhibition on voriconazole.Glycerol did not affect the test results of the five antifungal agents,and liquid paraffin on the test results of the five antifungal agents had little effect.Different concentrations of various vegetable oils and TWEEN had significantly inhibition on the antifungal activity of itraconazole(ITR),the MICs of the two reference strains were significantly higher(more than 8-32 times higher than the no lipidMICs).The four kinds TWEEN in different concentrations had different inhibition on the antifungal activity of amphotericin B(AMB) in different species.The inhibition was significantly on the two concentrations in C.krusei ATCC6258,and the inhibition was stronger in high concentrations than in low concentrations.Other types of lipid other than TWEEN had no inhibitory effect on amphotericin B(AMB).The test results on Malassezia furfur ATCC14521 showed that there was no growth in control wells which were adding no lipid,adding glycerol and TWEEN20.Every lipid had little effects on the results of voriconazole.And the influence was different on the results of fluconazole,amphotericin B and itraconazole. CONCLUSION Lipid has influence on the results of antifungal activity in vitro,the influence varies in different drugs,the impact depends on the lipid species with the lipid concentrations increasing as the MICs of drugs are increasing.Although glycerin has no influence on MICs of C.krusei ATCC6258 and C.parapsilosis ATCC22019,glycerin can promote the growth of the Malassezia furfur.therefore glycerin can not be used as the additive for the drug susceptibility testing of Malassezia.
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