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Study on the Factors That Influenced on Fast Propagation and Regeneration in Vitro of Fine-root Yam

SU Chao,LIU Pan-na,YUAN Jin-hong,SUN Ning-ning,XU Heng-jian(The Life College of The Shandong University of Technology,Zibo Shandong 255049)  
The system of shoot tip culture and regeneration in vitro of yam were established by plant tissue culture,and its influenced factors were explored.The results showed that:the calli were induced in the media with 6-BA 2.0 mg/L+NAA 0.5 mg/L.4-5 buds developed after the callus were transferred to the media with KT 2.5 mg/L+NAA 0.02 mg/L and KT 2.0 mg/L NAA 0.03 mg/L,and the shootlets grew strongly in the latter media.The media with KT 1.5 mg/L+NAA 0.02 mg/L showed good proliferation effect for leaf buds,and the proliferative ratio was to 3.8.The media with KT 2.0 mg/L+ IAA 0.2 mg/L was suitable for subculture.
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