Preliminary study on the antitumor immuno protective mechanism of β elemene

WU Weizhong, LIU Kangda, TANG Xiaolei , et al. Liver Cancer Institute, Zhongshan Hospital, Shanghai Medical University, Shanghai 200032  

Objective To study the antitumor effects and their molecular mechanism of β elemene, which is one of the effective monomers of Oleum Curcuma aromatica, a Chinese anticancer medicament.Methods The expression of membrane protein HSP70 on H22 cells treated with β elemene or mitomycin C(MMC) was analysed by immunofluorescence and flow cytometric techniques and the antitumor effects of the treated H22 cells was examined in C57BL/6 mice.Results The expression rate or intensity of membrane protein HSP70 of H22 cells treated with β elemene, and those treated with MMC was both significantly higher than that of the untreated H22 cells ( P 0.001). Increase in HSP70 expression was most marked when H22 cells were treated with β elemene and MMC in combination, with an expression rate of 95.1%. Heat shock treatment of the H22 cells combined with other stress inducing factors could further increase the HSP70 expression intensity. Specific antitumor immunty could be elicited in C57BL/6 mice immunized with β elemene treated H22 cells, and the effect could be partially blocked by anti HSP70 mAb. In mice immunized with β elemene treated H22 cells, tumor developed in 3 of 11 mice, while in the immunized mice given anti HSP70 mAb, 5 out of 10 mice developed tumor, and all of the control mice developed tumor. The average tumor weight (g) on day 28 after challenge with untreated H22 cells was 0.17±0.33, 0.66±0.77 and 1.11±0.58 in the three groups of mice, respectively.Conclusion β elemene increases tumor cell immunogenecity by inducing, at least in part, elevated expression of heat shock protein 70 on tumor cell surface.

【Key Words】： elemene Cancer vaccine Heat shock proteins
【Fund】： 卫生部科学研究基金资助;;上海市领先学科基金部分资助
【CateGory Index】： R73-36

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