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《Chinese Journal of Plastic Surgery and Burns》 2005-05
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Study on viability measurement of fat for grafting

LEI Hua, LI Qing-feng.Department of Plastic Surgery, the Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China  
Objective To establish a new method to accurately measure the viability of fat for grafting, which can lay a foundation for the further study of fat transplantation. Methods We centrifugated 5 groups of fat respectively at 1?000 r/min, 2?000 r/min, 3?000 r/min, 4?000 r/min and 5?000 r/min, took out 12 samples from per group of centrifugated fat, 5 ml per sample, and put them into dishes to incubate with DMEM containing glucose for one hour. Simultaneously we set up 5 control dishes (only containing DMEM without fat) to incubate together with those experimental dishes. After incubating we measured the glucose consistence of DMEM in all dishes. The glucose consistence difference between the mean value of 5 control dishes and per experimental dish represented the glucose transportation quantity of per sample, in other words, the sample viability. We analyzed and compared the glucose transportation quantity of every group of samples with t test. The 5 groups of fat were also put in histopathologic examination and the amounts of intact adipocytes on per group of slides were compared with T test. Results The glucose transportation quantities of group 1?000 r/min,2?000 r/min, 3?000 r/min, 4?000 r/min, 5?000 r/min gradually decreased in turn (P0.05). The histopathologic examination didn't show the difference among group 1?000 r/min, 2?000 r/min, 3?000 r/min and 4?000 r/min the 5?000 r/min (P0.05), and only showed the amounts of intact adipocytes on slides of group 5?000 r/min were significantly fewer than other four group (P0.05). Conclusion The glucose transportation test can be used to accurately measure the viability of fat. The histopathologic examination can reveal fat viability roundly. ;
【Fund】: 上海市科技发展基金资助项目(034119826)
【CateGory Index】: R622
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