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《CHINESE JOURNAL OF PARASITIC DISEASE CONTROL》 1998-04
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ESTABLISHMENT AND APPLICATION OF DOT IMMUNOGOLD FILTRATION ASSAY FOR DETECTION OF ANTI SCHISTOSOME ANTIBODIES

Ding Jianzu 1 Gan Xiaoxian 1 Shen Huiying 1 Shen Liying 1 Yu Jian 2 1 Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences(Hangzhou 310013) 2 The Second Peoples's Hospital of Huzhou , Zhejiang  
Dot immunogold filtration assay (DIGFA) was established to detect anti schistosome antibodies, using schistosome egg extract as antigen and rabbit anti human IgG labelled with colloidal gold as probe. Eighty six of eighty seven serun samples from patients infected with schistosome were positive. The positive rate was 98.9%. All of 100 sera of control from non endemic areas were negative. Only one out of 15 sera from patients with paragonimiasis showed positive. None of 10 sera from patients with clonorchiasis and 25 sera from patients with HBV were positive. DIGFA and ELISA had a similar sensitivity and specificity. The coincidence rate was 96.7%. However, DIGFA was simpler and easier to conduct and required no instrument. Therefore, this method is applicable for field investigation.
【CateGory Index】: R383.24,
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