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《Chinese Journal of Critical Care Medicine》 2007-09
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Effect of rhubard on heat shock protein 70 expression in rats with aute lung injury

JIN Kang, YU Dong - yun, YOU Tian. Department of Emergency, the First Hospital Affiliated to China Medical University, Shenyang 110001, China  
Objective To investigate the expression of HSP70 in acute lung injury induced by endotoxin lipopolysaccharide(LPS) and the protective mechanisms of rhubard against ALI.Methods LPS was injected into rats via the internal jugular vein to induce ALI and rhubard extract was given by intraperitoneal injection. The rats were randomly divided into four groups: saline control group, LPS-induced ALI group,ALI treated with prophylactic rhubard group, and ALI treated with therapeutic rhubard group.The rats were sacrificed 2 hours after injection of LPS or saline. Physiological and pathological examinations were conduced,including arterial gas analysis,lung coefficient,hematoxylin-eosin staining ,and HSP70 expression(immunohistochemistry and Western blotting). Results In the LPS-induced ALI group,interstitial edema,neutrophil infiltration,plasma exudation in alveoli, and damaged vascular endotheliun were observed.HSP70 levels were slightly increased in LPS-treated rats,while HSP70 expression was enhanced significantly and overall lung function was improved in rhubarb-treated groups compared to ALI model rats.Conclusions Our study confirme that rhubard protects rats from LPS-induced ALI and upergulates HSP70 expression.Thus,the protective effect of rhubard may be mediated by the HSP70 signaling pathway.
【CateGory Index】: R285.5
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