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《Acta Neuropharmacologica》 2011-01
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Establishment about Several Anti-oxidant Models of Microplate Quanti-fication Suitable for Drug-screening and Their Stability Testing

ZHANG Dan-shen Department of pharmacy,Hebei North Univesity,Zhangjiakou,075000,China  
Objective: To establish a series of anti-oxidant models of microplate quantification suitable for the drug screening.Methods: By using SpectraMax M5 continuous spectrum enzyme sign reflectoscope reflector,the anti-oxidant microplate quantification experiment modles of anti-DPPH oxidation activity,elimination hydroxy free radical,reduction ability determination,and lipid peroxidation were established.Results:All of the anti-oxidant microplate quantification experiment modles had more common characteristices of the good stability,the fewer reagent types and quantity,simple operation and step,the good repeatability,and so on.Relatively speaking,the two anti-oxidant modles of anti-DPPH oxidation activity,reduction ability determination had more advantage in these experiment modles,and had more suitable for initially screening experiment of the anti-oxidant drug as basic research.Conclusion: These four modles of the anti-oxidant microplate quantification may be widely applied in anti-drug screening,especially high-throughput screening research.
【Fund】: 河北省自然基金资助(No.2009001026)
【CateGory Index】: R965
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