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《Journal of Zhejiang Forestry College》 2008-03
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Optimization of AFLP molecular marker system in Lagerstroemia plants in China

GU Cui-hua1,WANG Shou-xian2,ZHANG Qi-xiang3(1.College of Landscape Architecture,Beijing Forestry University,Beijing 100083,China;2.School of Information Engineering,Zhejiang Forestry College,Lin'an 311300,Zhejiang,China;3.State Flower Engineering Center,Beijing Forestry University,Beijing 100083,China)  
This study dealt with optimization of amplified fragment length polymorphism(AFLP) analysis based on the high quality DNA extracted by the improved cetyl trimethyl ammonium bromide(CTAB) method from the leaves of Lagerstroemia indica,L.subcostata,L.limii and L.guilinensis which were gathered in Guangxi,Yunnan and Henan Province.And their DNA were used as the template to carry on restriction-ligase reaction,then the product was diluted the certain multiple as template to take pre-amplification,finally the product was diluted and used as template to carry on selective amplification,and the best system of restriction-ligase reaction,pre-amplication and silver staining suit for L.indica had been optimized.In the AFLP system each step of response had established different reacting system.The 10 pairs of primer suit for L.indica were selected from 160 pairs of elementary primer.The result indicated that,the best systems suitable for the Lagerstroemia gene group were as follows: restriction-ligase reaction system 1;pre-amplification system 3;selective amplification system 3,in reacting system the best Mg2+ concentration was 1.2×10-6 kg·L-1.
【Fund】: “十一五”国家科技攻关计划项目(2004BA525B11)
【CateGory Index】: S685.99
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