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《JOURNAL OF ZHEJIANG FORESTRY COLLEGE》 1999-02
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Fast propagation techniques of Beltilla ochracea by means of tissue cultures

ZHU Yuqiu1,WANG Xuegen2 (1.Department of Resources and Environment,Zhejiang Forestry College,Linan 311300,China;2.Jincheng Forest Station of Linan City,Linan 311300,China)HT  
A study of techniques for tissue cultures of Bletilla ochracea was made in terms of basic culture media,hormones and their proportions and the cutting of original bulbs.Its showed that the medium compose of 1/2 MS+10 mgL-1BA+01 mgL-1NAA was favorable to the proliferation of the original bulb,with 421 times asmany as thd original proliferated in 60 days;the medium consisting of Kyoto+10 mgL-1BA+01 mgL-1NAA was favorable to the induction of the original bulb to proliferate and differentiate and the growth of seedlings,with 479 times as many as the number of buds of the original bulb differentiated and formed within 60 days;and the medium with Kyoto+20 mgL-1BA+01 mgL-1NAA involved had a good effect on the bud induction of pieces cut from the original bulb.In the culture of subsequent generations,vertical cutting of the bulb or natural separation of the original bulb should be used.Some suggestions for further studies are put forward meanwhile.
【CateGory Index】: S682.310.38
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【References】
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