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《Acta Agriculturae Zhejiangensis》 2000-06
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Study on activity of acetolactate synthase (ALS) and inhibition of bispyribac sodium in rice and barnyardgrass:

WANG Qiang,WANG Yong,ZHAO Xue ping,WU Chang xing,DAI Fen( Institute of Plant Protection,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)  
Acetolactate synthase (ALS) is the main target of herbicides and bispyribac sodium prevents biosynthesis of branched chain amino acids by inhibition of ALS.In this paper,the activity of ALS was determined by an acetoin creatine naphthol complex on spectrophotometer and inhibition of bispyribac sodium to ALS in rice and barnyardgrass assessed.The results showed absorb peak of the complex for rice and barnyardgrass is 520 nm.Rice varieties Xianyou 63 and Xiushui 11 have the highest ALS activity,Jiayu 293 has the lowest one,and Xianhu 84 with the medium.For different weeds,the order of ALS activity from high to low is Echinochloa crusgalli,Eclipta prostrata,Cyperus difformis,Leptochloa chinensis. By in vivo test,ALS is prevented by bispyribac sodium and then recovered quicker in rice than in barnyardgrass.For rice varieties,ALS is repressed quickly in Jiayu 293,strongly in Xiushui 11,whereas in Xianhu 84 and Xianyou 63 the herbicide has slow and week inhibition to ALS.By in vitro test,the inhibition of bispyribac sodium to ALS is stronger than pyanchor.
【Fund】: 浙江省“九五”重大科技项目 !(96 110 2 2 42 )
【CateGory Index】: S481
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