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《Acta Agriculturae Zhejiangensis》 2019-02
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Isolation,identification of horse Bacillus cereus and its virulence genes detection

CUI Yilong;SHI Yun;YANG Dahan;YIN Youqin;XUE Jiangdong;HUO Xiaowei;MA Dehui;College of Animal Science and Technology,Inner Mongolia University for the Nationalities;Inner Mongolia Engineering Technology Research Center of Prevention and Control the Beef Cattle Disease;  
In order to identify a pathogenic bacterium isolated from the incidence and death racing of an equestrian club in Tongliao city,Inner Mongolia,the isolated strains were subjected to Gram stain microscopy,animal pathogenicity tests,automatic microbiological analysis system detection,16 S r RNA gene amplification,phylogenetic tree analysis and partial virulence gene PCR detection. The results showed that the biochemical characteristics of isolates were consistent with the characteristics of Bacillus cereus( B. cereus); it was sensitive to clindamycin,gentamicin and other drugs,moderately sensitive to cefoxitin,ciprofloxacin and other drugs,and resistant to aztreonam,cefazolin and other drugs; 16 S r RNA gene sequence was 100% homologous to the registered B. cereus sequence in Gen Bank; phylogenetic tree analysis showed that the isolated strains were closest to the relatives of B. cereus; the detection results of virulence gene PCR showed that 8 potential virulence genes ent FM,cyt K,hbl A,hbl C,hbl D,nhe A,nhe B and nhe C were all positive. The results showed that the isolated strain was a serious pathogenic B. cereus.
【Fund】: 国家自然科学基金(31460662);; 内蒙古自治区自然科学基金(2017MS0319)
【CateGory Index】: S852.61
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