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《Acta Agriculturae Zhejiangensis》 2019-02
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Gene cloning and bioactivity analysis of chitinase gene AO-483 from Arthrobotrys oligospora XJ-A1

GONG Shasha;MENG Qingling;QIAO Jun;ZHONG Wenqiang;HUANG Yunfu;ZHANG Guowu;CHEN Ying;CAI Xuepeng;Department of Animal Science & Technology,Shihezi University;Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences;  
In order to study the functions of chitinase,chitinase gene AO-483 of nematode-trapping fungi Arthrobotrys oligospora XJ-A1 was cloned,analyzed and expressed in prokaryotic expression system Escherichia coli. The pure prokaryotic expression recombinant fusion protein was purified by Ni column and the chitinase activity was determined by DNS method,and the biological activity of recombinant protein AO-483 was verified by dealing with larvae of Caenorhabditis elegans. The results showed that chitinase AO-483 gene encoded 309 amino acids,the homology of its gene sequence was 96. 88% and the amino acid sequence was 97. 73% respectively with Arthrobotrys oligospora standard strain( ATCC 24927). The chitinase AO-483 belonged to the family 18 glycoside hydrolase with a Glycohydro-18 structure domained at amino acid sequence of 20 ~ 297 and had two prevalent conserved catalytic domained with the sequence of GMLGG and LDGLDLDVE. The tertiary structure was a typical triosephosphate isomerase barrel structure. The protein identified by SDS-PAGE showed that the recombinant protein had a molecular weight of about52 ku which was consistent with prediction. Western blot revealed that recombinant protein can specifically react with anti-Arthrobotrys oligospora polyclonal antibody. The activity of recombinant chitinase AO-483 was determined by the DNS method,with a value of 223. 31 U·m L~(-1). The recombinant AO-483 owned stronger biological activity that can degrade Caenorhabditis elegans larvae stage 1 and stage 4.
【Fund】: 国家自然科学基金(31460654 31260601);; 新疆自治区研究生科研创新项目(XJGRI2015038)
【CateGory Index】: S852.731
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 ZHAO Hailong;MENG Qingling;QIAO Jun;GONG Shasha;HU Zhengxiang;LIU Tianli;CAI Xuepeng;CHEN Chuangfu;Department of Animal Science And Technology,Shihezi University;Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences;;Cloning and Bioinformatics Analysis of the Chitinase Gene from Arthrobotrys oligospora XJ-A1[J];西北农业学报;2015-03
2 WANG Jun-wei1,MENG Qing-ling1,QIAO Jun1,CAI Xue-peng2,LUO Jian-xun2, WANG Wei-sheng1,ZHANG Zai-chao1,CAI Kuo-jun1(1.Key Laboratory of Preventive Veterinary,College of Animal Science and Technology,Shihezi University,Shihezi 832003,China; 2.State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China);Culture conditions of Caenorhabditis elegans and its application in the study of nematode-trapping fungi[J];中国预防兽医学报;2012-10
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 ZHANG Xin;ZHAO Yong;TAN Haidong;MA Junyan;LI Kuikui;ZHANG Jianping;YIN Heng;Dalian Institute of Chemical Physics,Chinese Academy of Sciences;University of Chinese Academy of Sciences;Medical College of Dalian University;;Cloning and bioinformatic analysis of lpmo M1 from Magnaporthe oryzae[J];中国酿造;2015-11
2 SHU Fanfan;DAI Qi;SU Xijun;SU Zifeng;SU Hongyan;College of Agriculture and Biology Science, Dali University;;Screening of High-performance Biocontrol Strains of Nematode-trapping Fungi from Yak Dung in Plateau Pastures of Yunnan Province[J];大理学院学报;2014-12
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 LI You-quan,GUAN Gui-quan,PENG Yu-lü,HE Hai-ning,LUO Jian-xun,YIN Hong(Key Laboratory of Veterinary Parasitology of Gansu Province/State Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China);Study on the culture and preservation of Caenorhabditis elegans[J];中国兽医科学;2011-10
2 MENG Qing-ling1,2,WANG Wei-sheng1,WANG Jun-wei1,CAI Xue-peng2,LUO Jian-xun2*,CHEN Chuang-fu1,QIAO Jun1(1.Key Lab of Preventive Veterinary,College of Animal Science and Technology,Shihezi University,Shihezi 832000,China;2.State Key Lab of Veterinary Etiological Biology,Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China);Isolation,identification and predation activity of Arthrobotrys oligospora Xinjiang isolates[J];中国预防兽医学报;2011-03
3 FENG Jun-li, ZHU Xu-fen(Dept. of Biosciences, Zhejiang University, Hangzhou 310012, China);Molecular biology of microbial chitinase[J];浙江大学学报(农业与生命科学版);2004-01
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