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《Acta Agriculturae Zhejiangensis》 2019-02
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β-Conglycinin triggered IPEC-J2 cell damage through p38/JNK MAPK signaling pathway

PENG Chenglu;ZHANG Yu;XIA Xiaodong;HE Mengchu;SHU Yingshuang;FENG Shibin;LI Yu;WANG Xichun;LI Jinchun;WU Jinjie;College of Animal Science and Technology,Anhui Agricultural University;  
In this study,in vitro culture techniques were used to study the effects of different concentrations of 7 S( β-conglycinin) on IPEC-J2( intestinal epithelial cells in piglets). The experiment was divided into six groups,A( control),B( 1 mg·m L~(-1)7 S),C( 5 mg·m L~(-1)7 S),D( 10 mg·m L~(-1)7 S),E( 5 mg·m L~(-1)7 S + 1μmol·L~(-1) SP600125),F( 5 mg·m L~(-1)7 S + 1 μmol·L~(-1) SB202190). After 24 hours,the viability of the cells was measured by CCK-8 assay. The contents of NO,5-HT,IL-6 and IL~(-1)0 were detected by ELISA,the expression levels of pJNK,p-p38 and Bcl-2 proteins were detected by western blot,the relative expression of Bad,Bax,Bcl-2 and Caspase-3 m RNA was detected by PCR. The results showed that the viability of IPEC-J2 cells was decreased in groups C and D( P 0. 01). The viability of cells in groups E and F was significantly higher than that in group C( P 0. 05). 7 S promoted the secretion of inflammatory cytokines NO,5-HT,and IL-6 and decreased the secretion of IL~(-1)0,the addition of inhibitors decreased the secretion of cytokines NO,5-HT,and IL-6,and increased the secretion of IL~(-1)0. 7 S promoted the expression of p-JNK and p-p38 proteins and decreased the expression of Bcl-2 protein. The addition of inhibitors inhibited the expression of p-JNK and p-p38 proteins and increased the expression of Bcl-2 protein. The ratio of Bcl-2/Bax m RNA decreased with the increase of 7 S concentration,and the ratio of Bax/Bcl-xlincreased with the increase of 7 S concentration. The relative expression of Caspase-3 m RNA increased with the increase of 7 S concentration. On contrary,often adding inhibitors,the ratio of Bcl-2/Bax increased,the ratio of Bax/Bcl-xldecreased,and the relative expression of Caspase-3 decreased. Our results indicated that 7 S caused damage to the intestinal epithelial cells of piglets through the p38/JNK MAPK signaling pathway.
【Fund】: 科技部科技富民强县专项行动计划(国科发农〔2012〕745号);; 安徽省现代农业产业技术体系(AHCYJSTX-05/07)
【CateGory Index】: S816.4
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【Citations】
Chinese Journal Full-text Database 6 Hits
1 XING Xiu-ping;YANG Huan-huan;WEI Qing-yong;WU Li-fang;YAN Lei;QUAN Ya-nan;Animal Science and Technology College,Jilin Agricultural University;Liyang Aquatic Technology Company Limited;;Effect of soybean meal and extruded soy flour on growth performance and nutritional composition of carp[J];西北农林科技大学学报(自然科学版);2015-12
2 LI Jin-jie;SONG Zhi-pu;Henan Vocational College of Agriculture;Linying County Animal Health Authority;;Study on the Distribution of β-conglycinin in Gastro-intestinal Tissue of Piglets[J];中国畜牧兽医;2014-05
3 WANG Fu-hui,LI Ying-li,YANG Xiao-dong,WANG Xiao-ping,FAN Hong-li,SUN Xiao-zhi,WANG Feng,LIANG Jian-guang(Inner Mongolia muquanyuanxing feed Co.,Ltd.,Hohhot 010070);Effects of Fermented Soybean Meal on Growth Performance in Calves[J];中国奶牛;2013-02
4 LUAN Zhaoshuang SONG Juan HU Caihong (The Key Laboratory of Molecular Animal Nutrition,Institute of Feed Science,Zhejiang University,Hangzhou 310058,China);Inhibitors of Mitogen Activated Protein Kinase Pathways: Effects on Intestinal Morphology and Permeability of Weaner Piglets[J];动物营养学报;2013-01
5 LIU Shan-shan1,WU Xiao-xia1,JIANG Zhen-feng1,LI Wen-bin1(1.Key Laboratory of Soybean Biology of Ministry of Education,Soybean Research Institute,Northeast Agriculture University,Harbin,150030);ADVANCE IN SOYBEAN 7S GLOBULIN β-CONGLYCININ[J];大豆科学;2007-03
6 SUN Ze-wei , QIN Gui-xin , LOU Yu-jie(College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China);SOYBEAN ANTIGENS AND ITS INFLUENCE ON PIGLETS AND CALVES[J];动物营养学报;2005-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Nana;CAO Hongzhan;LU Chunlian;College of Animal Science and Technologe, Agricultural University of Hebei;;The Application of Fermented Soybean Meal in Livestock[J];饲料博览;2015-09
2 LI Bao;LI Yu;MA Liang-you;FENG Shi-bin;DING Hong-yan;WANG Xi-chun;WU Jin-jie;College of Animal Science and Technology,Anhui Agrigultural University;;Effects of soybean antigen protein on cytokines and the mRNA expression of tight junction protein Claudin-1 in intestinal epithelium of weaning piglets[J];中国兽医学报;2015-09
3 ZHAO Heng;LI Gui-quan;College of Agriculture,Shanxi Agricultural University;;Research on the Correlation of Storage Protein,Yield and Quality Characters of the Soybeans(Jinda 52 × Jinda 57)Hybrid Progeny Population[J];大豆科学;2014-05
4 LI Jin-jie;SONG Zhi-pu;Henan Vocational College of Agriculture;Linying County Animal Health Authority;;Study on the Distribution of β-conglycinin in Gastro-intestinal Tissue of Piglets[J];中国畜牧兽医;2014-05
5 WANG Zhang-cun,LI Le-jing,YUAN Dao-qiang,CUI Sheng-wen,ZHAO Xue-wei,WANG Ji-zhong,HU Jin-qiang(School of Food and Bioengineering,Zhengzhou University of Light Industry,Zhengzhou 450002,China);Changes in Molecular Subunit Composition and Allergenicity of Soybean Proteins during Enzymatic Hydrolysis[J];食品科学;2013-09
6 SONG Jia,CHEN Ye,ZHENG Xiao-chen,HAN Tao,JI Xu-qian,YU Cui-liu,KANG Jing-kun(College of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China);Study on Extraction Process Optimization of Soybean 11 S and 7 S Globulin[J];食品研究与开发;2013-02
7 SUN Zhi-kuo,WANG Xi-chun,SHI Nian-jin,WANG Yang, ZHONG Gang,XU Tong-jian,LIU Fang-fang,WU Jin-jie(College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China);Development of an indirect enzyme-linked immunosorbent assay for detecting serum antibodies of soybean antigen protein[J];畜牧与兽医;2013-01
8 DAI Jia-le,MA Jian,FU Yong-ping,QU Jing,WANG Pi-wu(Agronomy College,Jilin Agricultural University,Changchun 130118,Jilin,China);Construction of the RNAi Expression Vector of Soybean 7S Protein β-subunit Gene[J];大豆科学;2012-06
9 SI Xiao-Xia1 WANG Zhi2 HUO Fei1 SHI Tong-Lin3 FU Yue-Jun3 TIAN Huai-Dong1(1.College of Life Science,Shanxi University,Taiyuan 030006;2.Shanxi Institute of Crop Germplasm Resources,Taiyuan 030031;3.Institute of Biotechnology,Shanxi University,Taiyuan 030006);Optimization of Method for Extraction and Isolation of β-conglycinin from Soybean Seed[J];植物研究;2011-04
10 ZHANG Jian-zhong 1,LU Jian-jun 2,(1Wucheng Husbandry Bureau,Jinhua 321000,China;2 Feed Science Institute,Zhejiang University,Hangzhou 310029,China);Effects of soybean antigen protein on the growth performance of weaning piglets[J];浙江农业学报;2010-06
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LV Na;ZHAO Guo-kun;SUN Qiang;ZHANG Hong-xi;YIN Xiao-ping;ZHANG Mao-lan;ZHANG Jie;College of Biological and Environmental Sciences,Zhejiang Wanli University;College of Food Science and Engineering,Jilin Agricultural University;;Preparation of the Polyclonal Antibody against Flavobacterium columnaris and Identification of its Immunological Properties[J];中国畜牧兽医;2013-10
2 LUAN Zhaoshuang YAO Lili FU Zhenning SONG Juan HU Caihong*(Key Laboratory of Molecular Animal Nutrition of Ministry of Education,Feed Science Institute, Zhejiang University,Hangzhou 310058,China);Effects of Weaning Stress on Intestinal Morphology,Mucosal Barrier and p38 MAPK Signaling Pathway in Piglets[J];动物营养学报;2012-11
3 WANG Ji-teng1,2,HAN Tao2,HU Shui-xin2,et al(1.South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300;2.Fisheries School of Zhejiang Ocean University,Zhoushan 316004,China);The Influence of Replacing Fish Meal Partially in the Diet with Soybean Meal on Growth,Body Composition and Feed Utilization of Juvenile Giant Croaker Nibea japonica[J];浙江海洋学院学报(自然科学版);2011-04
4 WU Li-fang,ZHAO Han,QIN Gui-xin,YU Cheng-long,ZHAO Yuan,SUN LingCollege of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;Effects of Replacement of Fish Meal with Two Soybean Protein Sources on the Activities of Protease and Amylase in Cyprinus carpio[J];吉林农业大学学报;2011-02
5 LIU Xiang-he 1,2 ,YE Ji-dan 1,2 ,WANG Zi-jia 1,2 ,WANG Kun1,KONG Jiang-hong1 (1.Fisheries College,Jimei University,Xiamen 361021,China; 2.Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation,Xiamen 361021,China);Partial replacement of fish meal by soybean meal in diets for juvenile Japanese flounder (Paralichthys olivaceus)[J];水产学报;2010-03
6 LU Yang YANG Yuhong WANG Yuyu WANG Lei WANG Ruixia(College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China);Effects of Different Replacement Ratio of Fish Meal by Extruded Soybean Meal on Growth,Body Composition and Hematology Indices of Rainbow Trout(Oncorhynchus mykiss)[J];动物营养学报;2010-01
7 Liu Yong1 Leng Xiangjun1 Li Xiaoqin1 Li Hui2,3 Liu Aijun1 (Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Education1,Shanghai 201306) (NingHai County Oceanic And Fishery Bareau2,Ningbo 315600) (Economic management department of Shanghai Ocean University3,Shanghai 201306);Effects of Replacing Fish Meal with Soybean Meal on Growth,Digestibility and Immunity of Tilapia (Oreochromis niloticus×O.aureus)[J];中国粮油学报;2009-12
8 Li Jian;Research and Development Progress of Fermented Soybean Meal[J];粮食与饲料工业;2009-06
9 WANG Chang-an1,ZHOU Chang-hai2,XU Qi-you1,XU Hong1,JIANG Shu-yi,ZHENG Qiu-shan 1,SUN Da-jiang1(1.Heilongjiang River Fisheries Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China; 2.Fuyuan Country Fisheries Technology Extension Station of Heilongjiang province,Fuyuan 156429,China);Effects of replacement of soybean meal with extruded soybean on the growth performance and immunity in hybrid sturgeon Acipenser schrenckii♀ × A.baeri♂[J];大连水产学院学报;2009-01
10 WU Li-fang,QIN Gui-xin,ZHANG Dong-ming,WANG Hong-he,SUN Ze-wei,SUN Ling (College of Animal Science and Technology,Jinlin Agricultural University,Changchun,Jilin 130118,China);Effect of dietary soybean protein level on the growth performance of carp and nutritional components in the muscle[J];西北农林科技大学学报(自然科学版);2008-10
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