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《Acta Agriculturae Zhejiangensis》 2019-02
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β-Conglycinin triggered IPEC-J2 cell damage through p38/JNK MAPK signaling pathway

PENG Chenglu;ZHANG Yu;XIA Xiaodong;HE Mengchu;SHU Yingshuang;FENG Shibin;LI Yu;WANG Xichun;LI Jinchun;WU Jinjie;College of Animal Science and Technology,Anhui Agricultural University;  
In this study,in vitro culture techniques were used to study the effects of different concentrations of 7 S( β-conglycinin) on IPEC-J2( intestinal epithelial cells in piglets). The experiment was divided into six groups,A( control),B( 1 mg·m L~(-1)7 S),C( 5 mg·m L~(-1)7 S),D( 10 mg·m L~(-1)7 S),E( 5 mg·m L~(-1)7 S + 1μmol·L~(-1) SP600125),F( 5 mg·m L~(-1)7 S + 1 μmol·L~(-1) SB202190). After 24 hours,the viability of the cells was measured by CCK-8 assay. The contents of NO,5-HT,IL-6 and IL~(-1)0 were detected by ELISA,the expression levels of pJNK,p-p38 and Bcl-2 proteins were detected by western blot,the relative expression of Bad,Bax,Bcl-2 and Caspase-3 m RNA was detected by PCR. The results showed that the viability of IPEC-J2 cells was decreased in groups C and D( P 0. 01). The viability of cells in groups E and F was significantly higher than that in group C( P 0. 05). 7 S promoted the secretion of inflammatory cytokines NO,5-HT,and IL-6 and decreased the secretion of IL~(-1)0,the addition of inhibitors decreased the secretion of cytokines NO,5-HT,and IL-6,and increased the secretion of IL~(-1)0. 7 S promoted the expression of p-JNK and p-p38 proteins and decreased the expression of Bcl-2 protein. The addition of inhibitors inhibited the expression of p-JNK and p-p38 proteins and increased the expression of Bcl-2 protein. The ratio of Bcl-2/Bax m RNA decreased with the increase of 7 S concentration,and the ratio of Bax/Bcl-xlincreased with the increase of 7 S concentration. The relative expression of Caspase-3 m RNA increased with the increase of 7 S concentration. On contrary,often adding inhibitors,the ratio of Bcl-2/Bax increased,the ratio of Bax/Bcl-xldecreased,and the relative expression of Caspase-3 decreased. Our results indicated that 7 S caused damage to the intestinal epithelial cells of piglets through the p38/JNK MAPK signaling pathway.
【Fund】: 科技部科技富民强县专项行动计划(国科发农〔2012〕745号);; 安徽省现代农业产业技术体系(AHCYJSTX-05/07)
【CateGory Index】: S816.4
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