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《Journal of Zhejiang University(Agriculture and Life Sciences)》 2010-03
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Extraction and purification of polyphenolic compounds and determination of EGCG from eagle tea (Litsea coreana Lévl. var. lanuginosa)

SHEN Jun-zi1,Ye Ming1,SHU A-qing2,YIN Ke-quan2,YANG Liu1(1.College of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China;2.Ningguo Yibang Biotechnology Co.Ltd.,Ningguo,Anhui 242300,China)  
The polyphenolic compounds were prepared using reflux extraction and polyamide chromatography from the powder of eagle tea(Litsea coreana Lévl.var.lanuginosa).Polyphenolic compounds were extracted with the 80% ethanol for three times at 100 ℃,of which,the extraction time was 30,20,10 min and the ratio of material to liquid was 1:20,1:10,1:10 respectively,and the crude extraction rate reached 31.46%.Then,the supernatant was collected after adjusting the pH of crude extract to 2.0 and centrifugating at 12 000 r·min-1 for 10 min,the yield rate of polyphenolic compounds from which was 93.59%.Finally,the supernatant was diluted to 2 mg·mL-1 and its pH was adjusted to 2.0 as the sample solution,the optimum conditions for purification of polyphenolic compounds using polyamide resin were as follows:the ratio of column height to diameter was 22 with sample volume at 1.5 BV and 1 h of adsorption equilibrium time,and the eluent was little deionized water and 80% ethanol successively with 6 mL·min-1 of eluent flow rate.Under the optimal conditions,the desorption rate of polyphenolic compounds reached 97.20%.The purity and extraction rate of polyphenolic compounds which were attained by freeze vacuum drying from concentrated eluent reached 92.80% and 18.06%,respectively.The epigallocatechin gallate(EGCG) content of polyphenolic compounds measured by HPLC was 49.82%.
【Fund】: 安徽省企业技术创新资助项目(2008991/QTXM0813)
【CateGory Index】: TQ914.1
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