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《Journal of Zhejiang Agricultural University》 1999-01
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Evolution of baculovirus occlusion genes Journal of Zhejiang Agricultural University

Zhang Chuanxi 1; Gong Chenliang 1; Hu Cui 1; Wu Xiangfu 2 (1 Institute of Applied Entomology, Zhejiang University, Hangzhou 310029, China ; 2 Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai 200031, China)  
The polyhedrin genes of HaSNPV and EoSNPV were sequenced,and the deduced amino acid sequences were compared with other occlusion body proteins from 26 baculoviruses by using MALIGN program The result shows that HaSNPV polyhedrin is most closely related to that of HzSNPV, with 97 6% aminoacid identity and 97 4% nucleotide identity, respectively; EoSNPV polyhedrin gene is most closely related to that of OpSNPV, with 83 0% nucleotide and 94 7% amino acid identity, and it also shares a high degree of homology with 20 other lepidopteran NPVs, with identities ranging from 72 6% to 81 9% for nucleotide sequence and 83 3% to 93 9% for amino acid sequences, respectively The relationship between EoSNPV polyhedrin and granulins is closer, with amino acid identities of 48 4% to 54 9%, than that between the polyhedrin of EoSNPV and the polyhedrin of NsSNPV, a Hymenopteran NPV, with only 44 5% amino acid identity Phylogenetic analysis of the polyhedrin gene shows that EoSNPV and HaSNPV are members of the previously described lepidopteran NPV group Ⅱ
【Fund】: 国家自然科学基金 浙江省自然科学基金
【CateGory Index】: S476
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