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《Chinese Journal of Parasitology and Parasitic Diseases》 2013-01
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Separation and Identification of the Surface and Secreted Proteins in Toxoplasma gondii by Biotin Labeling and Proteomics Methods

LIU Yuan~1,XUE Feng~(1,2),HUANG Min-jun~(1,2),GAN Shao-bo~(1,2),GU Jun-chao~(1,2*) (1 Beijing Tropical Medicine Research Institute,Beijing Friendship Hospital Affiliated to the Capital Medical University,Beijing 100050,China;2 Beijing Key Laboratory for Tropical Disease Control,Beijing 100050,China)  
Objective To separate and identify the surface proteins and secreted proteins of Toxoplasma gondii tachyzoties of RH strain.Methods T.gondii tachyzoties were cultured in Vero cells,and purified by filtration and Percoll cell separation solution.The biotin-labeled tachyzoites were lysed,and the surface and secreted proteins were separated by NeutrAvidin agarose beads.After condensation and SDS-PAGE,the protein were collected,digested and identified by LC/MS-MS.Results A total of 785 T.gondii proteins were identified,81(10.3%) of which were originally annotated as the surface or secreted proteins.Among the highly-expressed(PSM10) 65 proteins,43(66%) were originally annotated as surface or secreted proteins,while the others were predicted unknown proteins.Conclusion The surface and secreted proteins of T.gondii are separated by biotin labeling and avidin chromatography,among which some potential new surface or secreted proteins of T.gondii are identified.
【Fund】: 国家自然科学基金(No.81071375);; 首都医科大学附属北京友谊医院科研启动基金项目(No.2011_30yykyqd);; 北京市自然科学基金(No.7132042)~~
【CateGory Index】: R382.5
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