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Biolistic mediated transformation and transient expression of Gus gene in leaves of Citrus medica L.var.sarcodactylis (Noot) Swingle

GUO Wei-dong~1, ZHOU Chun-li~1,2, LU Mei~1, XIE Xuan-song~1(1.College of Chemistry and Life Science,Zhejiang Normal University,Jinhua Zhejiang 321004,China;2.Northwest Sci-Tech Uniwersity of Agriculture and Forestry,Yangling Shanxi 712100,China)  
Tender and elder leaf-discs of fingered-citrus(Citrus medica L.var.sarcodactylis(Noot) Swingle) were transformed with Gus gene via biolistics method.The transient expression of Gus gene was used to analyse the transformation rate under different target distance.The results indicated that a higher transformation rate according to transient expression of Gus gene could be acquired from tender leaf-discs bombarded under 7.584 5×10~6 Pa rupture pressure and 6 cm target distance.Transformation rate of elder-leaf was lower.Increasing bombardment times could not improve the transformation rate.
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