Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Zhejiang Normal University (Natural Sciences)》 2005-04
Add to Favorite Get Latest Update

Biolistic mediated transformation and transient expression of Gus gene in leaves of Citrus medica L.var.sarcodactylis (Noot) Swingle

GUO Wei-dong~1, ZHOU Chun-li~1,2, LU Mei~1, XIE Xuan-song~1(1.College of Chemistry and Life Science,Zhejiang Normal University,Jinhua Zhejiang 321004,China;2.Northwest Sci-Tech Uniwersity of Agriculture and Forestry,Yangling Shanxi 712100,China)  
Tender and elder leaf-discs of fingered-citrus(Citrus medica L.var.sarcodactylis(Noot) Swingle) were transformed with Gus gene via biolistics method.The transient expression of Gus gene was used to analyse the transformation rate under different target distance.The results indicated that a higher transformation rate according to transient expression of Gus gene could be acquired from tender leaf-discs bombarded under 7.584 5×10~6 Pa rupture pressure and 6 cm target distance.Transformation rate of elder-leaf was lower.Increasing bombardment times could not improve the transformation rate.
【Fund】: 浙江省科技计划项目资助项目(2003C32043)
【CateGory Index】: S686
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 9 Hits
1 CAO Ying,HU Shang lian,LI Wen xiong,LIU Jin hong (Northeast Agricultural University,Harbin,Heilongjiang,150030,PRC);Studies on Bar gene transformation of immature embryos in spring wheat (Triticum aestivum L.) by particle bombardment --The important factors effected on transformation[J];Journal of Northeast Agricultural University;2003-01
2 LIU Weihua1,2,LI Wenxiong1,HU Shanglian1,XU Xiangling2,LI Jilin2,CHEN Zhenghua3,HU Zanmin3(1 Department of Agronomy,Northeast Agricultural University,Harbin 150030,China;2 Department of Biology,Harbin Normal University,Harbin 150080,China;3 Institute of Genetics,Chinese Academy of Sciences,Beijing 100101,China);Studies on transferring chitinase gene into wheat via microprojectile bombardment[J];Acta Botanica Boreali-occidentalia Sinica;2003-01
3 CHEN Bing-chu,SHENTU Wen-yue(College of Life and Environment, Zhejiang Normal University,Jinhua321004,Zhejiang,China);Compared on Biological Characteristics in 3 Types of Jinhua Fingered Citron[J];Forest Research;2003-06
4 ZHAO Yan 1~3 ,YU Yan Chun 1,3 ,QIAN Qian 3,① ,YAN Mei Xian 3,HUANG Da Nian 1,3,② (1 College of life science,Zhejiang University,Hangzhou 310029,China; 2 Biological engineering Department,Hangzhou University of Commerce,Hangzhou 310035,China; 3 Biotechnology Department,Key Laboratory for Rice Biology,Ministry of Agriculture,China National Rice Institute,Hangzhou 310006,China);Cotransformation of Rice by bar and cecropin B Gene Expression Cassettes Lacking Vector Backbone Sequences[J];Acta Genetica Sinica;2003-02
5 CHEN Bing-chu, ZHAO Bin, SHENTU Wen-yue ( 1. College of Chemistry and Life Sciences,Zhejiang Normal Universily , Jinhua Zhejiang 321004 ,China ;2. Zhejiang Senhe Seed Co. Ltd,Hangzhou Zhejiang 310020,China);Research on medium culture technology and some physiological index of Jinhua fingered citron[J];Journal of Zhejiang Normal University (Natural Sciences);2003-03
6 ZHENG Xiao-hua, WENG Xue-xiang(College of Chemistry and Life Science,Zhejiang Normal University,Jinhua Zhejiang 321004,China);Determination of volatile components in bergamot by gas chromatography-mass spectrometry with solid-phase microextraction[J];Journal of Zhejiang Normal University (Natural Sciences);2004-01
7 Zhu bing Huang Danian Yang Wei Xue Rui Xiao Han (China National Rice Research Institute, Hangzhou 310006) Tian Wenzhong Li Liangcai Dai Shunhong (Instituete of genetics, Academic Sinica);Prodution of Herbicide resistant Transgenic Rice Plants from Immature Embryos Using Biolistic Method[J];SCIENTIA AGRICUTURA SINICA;1996-06
8 Jin Xiaoing(Zheiang Normal University,Jinhua 321004).;The Exploitation and Utilization of Jindua Bergamot Resource[J];RESOURCE DEVELOPMENT & MARKET;1998-06
9 WU Shan 1, , LIANG Yue Rong 1,ZHU Yun Guo 2 ( 1Department of Tea Science, Zhejiang University, Hangzhou 310029; 2Scientz Institute of Instruments, Ningbo, Zhejiang 315010 );Application of Particle Bombardment in the Research of Plant Genetic Engineering[J];Plant Physiology Communications;2003-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Chen Jiahua, Lin Zuming, fin Sheng (Department of Chemistry);The Study of Chemical Compositions of Top Note of Bergamot[J];Acta Scicentiarum Naturalum Universitis Pekinesis;1989-02
2 Chen Shouyi,Peng Yuji,Huang Qiong,Yang Fei(Guizhou Province sweet orange research institute,Luodian 550100);Study of the decorative and medicinal values of two varieties of Citrus medica[J];Forest By-Product and Speciality in China;2006-06
3 Guo Tianchi (Citrus Research Institute,Chinese Academy of Agricultural Sciences,Chongqing 630712);Citron in China[J];;1993-04
4 Cheng Zhongping1,2, Chen Zhiwei2 , Hu Chungen3, Deng Xiuxin3,and Luo Zhengrong3 (1Wuhan Forestry and Fruit Research Institute,Wuhan 430075 2Wuhan Institute of Botany,CAS,Wuhan 430074; 3State Key Laboratory in Genetic Improvement of C;Analysis of Special Cultivar Germplasm in Peach by RAPD Markers[J];Journal of Fruit Science;2001-04
5 Chen Bingchu,Zhao Tieqiao,Ma Bojun,and Shao Chunrong(College of Life and Environment, Zhejiang Normal University, Jinhua, Zhejiang 321004 China; Zhejiang Senhe Seed Co. Ltd ,Hangzhou,Zhejiang 310010 China);Type Delimitation and RAPD Analysis of Jinhua Fingered Citron (Circo-dactylitrus medica var. sarcodactylis Swingle)[J];Journal of Fruit Science;2002-04
6 Zhang Qinglin and Luo Zhengrong  (College of Horticulture and Forestry,Huazhong Agricultural University,Wu han,Hubei430070China);ISSR Technology and Its Applications in Fruit Trees[J];Journal of Fruit Science;2004-01
7 XU Jian, LI Guo hui, ZHOU Yu ping, TIAN Chang en, ZHOU Bo chun, WANG Zheng xun ( Department of Biology, Guangzhou University , Guangzhou 510405, China );Effect of La(NO_3)_3 on the POD activity and relative conductivity of leaves in banana seedlings under chilling condition[J];Guihaia;2002-03
8 YANG Yu-ling~(1,2),MA Xiang-qing~2,ZHANG Mu-qing~1 (1.Key Lab of Eco-physiology & Genetic Improvement for Sugarcane,Ministry of Agriculture,P.R.China;2.College of Forestry,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);ISSR molecular marker and its application to the research on genetic breeding of trees[J];Subtropical Agriculture Research;2006-01
9 Huang Xiaoyu Zhong Xiuyin Su Yi (Dept. of Food Science, South China Agric. Univ.,Guangzhou, 510642);EXTRACTION AND INDENTIFICATION OF VOLATILE OIL IN FINGERED CITRON[J];JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY;1998-03
10 Li Xiuju ( Agrionomy Department) Ding Zhongrong (Basic Course Department, Northwestern Agricultural University);The Effect of Cold Hardening on Plasma Membrane and Protective Enzyme in Different Winter Wheat Cultivars[J];Journal of Henan Vocation-technical Teachers College;1991-02
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 XU Xiaobo,JIANG Donghua,LI Jie,HOU Jiaheng(College of Chemistry and Life Science,Zhejiang Normal University,Jinhua Zhejiang 321004,China);Genetic diversity analysis with ISSR PCR on different yeasts[J];Journal of Zhejiang Normal University(Natural Sciences);2010-01
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Chen Jiahua, Lin Zuming, fin Sheng (Department of Chemistry);The Study of Chemical Compositions of Top Note of Bergamot[J];Acta Scicentiarum Naturalum Universitis Pekinesis;1989-02
2 Chen Bingchu, Zao Qing, and Shentu Wenyue(Biology Department of Zhejiang Normal University, Jinhua 321004);Comparison of the Cold Resistance of Jinhua Fingered Citron Grafted on Different Rootstocks[J];SOUTH CHINA FRUITS;1997-02
3 Chen Bingchu,Zhao Tieqiao,Ma Bojun,and Shao Chunrong(College of Life and Environment, Zhejiang Normal University, Jinhua, Zhejiang 321004 China; Zhejiang Senhe Seed Co. Ltd ,Hangzhou,Zhejiang 310010 China);Type Delimitation and RAPD Analysis of Jinhua Fingered Citron (Circo-dactylitrus medica var. sarcodactylis Swingle)[J];Journal of Fruit Science;2002-04
4 Mi Qingli He Sijie Cao Shouyun Li Liangcai Tian Wenzhong (Institute of Genetics, Academia Sinica, Beijing 100101);Differences in Response to Biolistic Transformation between Indica and Japonica Rice and the Way to Increase Their Efficiency[J];JOURNAL OF AGRICULTURAL BIOTECHNOLOGY;1998-03
5 JIN Xiao _ ling, XU Li _ shan, ZHENG Xiao _ hua(Department of Biology, Zhejiang Normal University, Jinhua 321004, China);Chemical Compositions of Essential Oil from Bergamot[J];JOURNAL OF INSTRUMENTAL ANALYSIS;2000-04
6 Chen Lianghong Wang Xinwang Zhang Wenjun Zhang Xiaodong Hu Daofen Liu Guangtian(Dept. of Plant Genetics and Breeding, China Agricultural University, Beijing, 100094; Institute of Genetics, Chinese Academy of Sciences, Beijing, 100101; Beijing Academy of Agricultural Sciences, Beijing, 100081);Transformation of Genes Encoding High Molecular Weight Glutenin Subunit (HMW-GS)into Common Wheat (Triticum aestivum L. )[J];Acta Agronomica Sinica;1999-04
7 Xu Xinping Hu Ming Yi Lezhou Wei Jianwen Li Baojian(Biotechnology Research Center, Zhongshan University, Guangzhou 510275);Efficient Production of Fertile Transgenic Rice Following Particle Bombardment[J];Acta Agronomica Sinica;1999-06
8 DAI Shun-hong LI Liang-cai DING Yue-yun HE Si-jie CAO Shou-yun TIAN Wen-zhong (Institute of Genetics Chinese Academy of Sciences Beijing 100101);Multiple Gene Transformation of Rice Using the Biolistic Method[J];Acta Genetica Sinica;1998-04
9 LIANG Hui ZHAO Tie-han LI Liang-cai OUYANG Jun-wen TIAN Wen-zhong JIA Xu (Institute of Genetics Chinese Academy of Sciences Beijing 100101);Studies on Some Factors Affecting Transformation of Wheat Immature Embryos by Biolistic Bombardment[J];Acta Genetica Sinica;1998-05
10 HUA Zhi-Hua1, ZHU Xue-Feng1, LIN Hong-Sheng 1,2, GAO Zhen-Yu1, QIAN Qian1, YAN Mei-Xian1, HUANG Da-Nian1 (1. Biotechnology Department, China National Rice Research Inst itute and Key Laboratory for Rice Biology, Ministry of Agriculture, Hangzhou 3;Studies of the Integration and Expression of Exogenes in Transgenic Rice Obtained via Particle Bombardment Transformation[J];Acta Genetica Sinica;2001-11
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved