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《The Orthopedic Journal of China》 2004-09
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Study on rabbit osteoblasts isolated and digested by collagenase in DMEM containing fetal bovine serum(FBS)

CHEN Chao,LI Guang hui,CHEN An min Orthopedic Department,Tongji Hospital,Tongji Medical College,Huazhong University of science and Technology,Wuhan 430030  
Objective:To investigate the cell culture technique of rabbit cranial osteoblasts in vitro and digested by type Ⅰ collagenase in DMEM containing FBS Method:The pregnat rabbits were killed The upper skull tissue of 28 day old fetal rabbit were isolated and digested by collagenase of different concentrations in DMEM containing FBS The isolated cells were then suspended in DMEM containing FBS The cell suspension were cultured in monolayer cultures The cell viability was assessed by staining with Trypan blue And the osteoblast purity was assessed by staining with Alkaline phosphatase (ALP) in cell Result:When rabbit osteoblasts were digested and cultured by collagenase in DMEM containing FBS,their rejective staining rate with Trypan blue was 98%,and their positive staining region not less than 95% with ALP;But their rejective staining rate with Trypan blue was 95% in control group,and their positive staining region not less than 75% with ALP in control group Conclusion:The rabbit osteoblasts digested and cultured by collagenase in DMEM containing FBS have typical characteristics of osteoblast with a single component,adequate osteoblast purity and proliferation
【CateGory Index】: R329.2
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